Fabrication of highly sensitive gold nanourchins based electrochemical sensor for nanomolar determination of primaquine

• Published in: Materials Science & Engineering C 2017-05, Vol.74, p.27-35
• Main Authors: Thapliyal, Neeta Bachheti, Chiwunze, Tirivashe Elton, Karpoormath, Rajshekhar, Cherukupalli, Srinivasulu
• Format: Article
• Language: English
• Subjects: Antimalarial agents; Antimalarials - analysis; Antimalarials - urine; Dielectric Spectroscopy; Electrochemical analysis; Electrochemical impedance spectroscopy; Electrochemical Techniques; Electrochemistry; Electrodes; Electron microscopy; Electron transfer; Fabrication; Formulations; Glassy carbon; Glassy carbon electrode; Gold; Gold - chemistry; Gold nanourchins; Human urine; Humans; Limit of Detection; Materials science; Metal Nanoparticles - chemistry; Microscopy, Electron, Scanning; Microscopy, Electron, Transmission; Oxidation; Pharmaceutical formulations; Primaquine; Primaquine - analysis; Primaquine - urine; Quality control; Reproducibility; Reproducibility of Results; Scanning electron microscopy; Selectivity; Sensors; Spectroscopy; Transmission electron microscopy; Urine; Voltammetry; Wave dispersion
• ISSN: 0928-4931; 1873-0191
• DOI: 10.1016/j.msec.2016.12.126

A gold nanourchins modified glassy carbon electrode (AuNu/GCE) was developed for the determination of antimalarial drug, primaquine (PQ). The surface of AuNu/GCE was characterized by electrochemical impedance spectroscopy (EIS), scanning electron microscopy (SEM), transmission electron microscopy (TEM) and cyclic voltammetry (CV). EIS results indicated that the electron transfer process at AuNu/GCE was faster as compared to the bare electrode. The SEM and TEM image confirmed the presence and uniform dispersion of gold nanourchins on the GCE surface. Upon investigating the electrochemical behavior of PQ at AuNu/GCE, the developed sensor was found to exhibit high electrocatalytic activity towards the oxidation of PQ. Under optimal experimental conditions, the sensor showed fast and sensitive current response to PQ over a linear concentration range of 0.01–1μM and 0.001–1μM with a detection limit of 3.5nM and 0.9nM using differential pulse voltammetry (DPV) and square wave voltammetry (SWV), respectively. The AuNu/GCE showed good selectivity, reproducibility and stability. Further, the developed sensor was successfully applied to determine the drug in human urine samples and pharmaceutical formulations demonstrating its analytical applicability in clinical analysis as well as quality control. The proposed method thus provides a promising alternative in routine sensing of PQ as well as promotes the application of gold nanourchins in electrochemical sensors. A gold nanourchins modified glassy carbon electrode was fabricated and used as an electrochemical sensing platform for the determination of primaquine. [Display omitted] •Gold nanourchins based electrochemical sensor for determination of primaquine•A detection limit of 0.9nM was obtained using square wave voltammetry.•Proposed method was applied to quantify the drug in tablet and human urine samples.•Fast, simple and low-cost method for trace analysis of primaquine