Relationships between body temperatures and inflammation indicators under physiological and pathophysiological conditions in pigs exposed to systemic lipopolysaccharide and dietary deoxynivalenol

• Published in: Journal of animal physiology and animal nutrition 2018-02, Vol.102 (1), p.241-251
• Main Authors: Tesch, T., Bannert, E., Kluess, J., Frahm, J., Hüther, L., Kersten, S., Breves, G., Renner, L., Kahlert, S., Rothkötter, H.‐J., Dänicke, S.
• Format: Article
• Language: English
• Subjects: Animal Feed - analysis; Animals; Bioindicators; Biomarkers - blood; Blood; Body temperature; Body Temperature - drug effects; Deoxynivalenol; Diet; Inflammation; Inflammation - chemically induced; Inflammation - metabolism; Inflammation - veterinary; Kynurenine - blood; lipopolysaccharide; Lipopolysaccharides; Lipopolysaccharides - toxicity; Markers; pathophysiological conditions; physiological; pig; Pigs; Sodium chloride; Swine; Temperature; Temperature effects; Trichothecenes - administration & dosage; Trichothecenes - toxicity; Tryptophan; Tryptophan - blood; Tumor necrosis factor; Tumor necrosis factor-TNF; Tumors
• ISSN: 0931-2439; 1439-0396
• DOI: 10.1111/jpn.12684

Summary We studied the constancy of the relationship between rectal and intraabdominal temperature as well as their linkage to inflammatory markers (leucocyte counts, kynurenine‐to‐tryptophan ratio (Kyn–Trp ratio), tumour necrosis factor alpha (TNF‐α) in healthy and in pigs exposed to lipopolysaccharide (LPS) and/or deoxynivalenol (DON). Barrows (n = 44) were fed 4 weeks either a DON‐contaminated (4.59 mg DON/kg feed) or a control (CON) diet and equipped with an intraabdominal temperature logger and a multicatheter system (V.portae hepatis, V.lienalis, Vv.jugulares) facilitating infusion of 0.9% NaCl (CON) or LPS (7.5 μg/kg BW) and simultaneous blood sampling. Body temperatures were measured and blood samples taken every 15 min for leucocyte counts, TNF‐α and Kyn–Trp ratio. Combination of diet and infusion created six groups: CON_CONjug.‐CONpor., CON_CONjug.‐LPSpor., CON_LPSjug.‐CONpor., DON_CONjug.‐CONpor., DON_CONjug.‐LPSpor., DON_LPSjug.‐CONpor.. The relationship between both temperatures was not uniform for all conditions. Linear regression revealed that an intraabdominal increase per 1°C increase in rectal temperature was ~25% higher in all LPS‐infused pigs compared to NaCl‐infusion, albeit diet and site of LPS infusion modified the magnitude of this difference. Inflammatory markers were only strongly present under LPS influence and showed a significant relationship with body temperatures. For example, leucocyte counts in clinically inconspicuous animals were only significantly correlated to core temperature in DON‐fed pigs, but in all LPS‐infused groups, irrespective of diet and temperature method. In conclusion, the gradient between body core and rectal temperature is constant in clinically inconspicuous pigs, but not under various pathophysiological conditions. In the latter, measurement of inflammatory markers seems to be a useful completion.