Development of high throughput LC/MS/MS method for analysis of perfluorooctanoic acid from serum, suitable for large-scale human biomonitoring

• Published in: Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2017-04, Vol.1049-1050, p.24-29
• Main Authors: Rogatsky, Eduard, O’Hehir, Colleen, Daly, James, Tedesco, Amelie, Jenny, Richard, Aldous, Kenneth
• Format: Article
• Language: English
• Subjects: Caprylates - blood; Chromatography, Liquid - methods; Environmental Exposure - analysis; Environmental Monitoring - methods; Fluorocarbons - blood; High throughput; Humans; LC/MS; Limit of Detection; Linear Models; PFOA; Reproducibility of Results; Sample preparation; Tandem Mass Spectrometry - methods
• ISSN: 1570-0232; 1873-376X
• DOI: 10.1016/j.jchromb.2017.02.020

A simple method for determination of perfluorooctanoic acid (PFOA) from human serum by liquid chromatography/tandem mass spectrometry (LC/MS/MS) and utilizing high-throughput sample preparation was developed by New York State Public Health Emergency Preparedness Laboratory and used for biomonitoring studies. 50μl of serum was mixed with a 0.4ml of acetonitrile containing isotopically labeled internal standard, followed by phospholipid removal/protein precipitation. The extract was partially dried and analyzed by LC/MS/MS. The linear range of PFOA analysis was 0.5–100ng/ml. LLOQ was selected as 0.5ng/ml. The method was validated following APHL guidelines for LRN-C laboratories and about 6000 specimens were successfully prepared and analyzed using this simple LC/MS method. Due to sample preparation simplicity, the presented method can be used in large-scale clinical testing, such as public health surveillance studies.