Bioprospection of cold‐adapted yeasts with biotechnological potential from Antarctica

The aim of this study was to investigate the ability to produce extracellular hydrolytic enzymes at low temperature of yeasts isolated from 25 de Mayo island, Antarctica, and to identify those exhibiting one or more of the evaluated enzymatic activities. A total of 105 yeast isolates were obtained f...

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Bibliographic Details
Published in:Journal of basic microbiology 2017-06, Vol.57 (6), p.504-516
Main Authors: Martorell, María Martha, Ruberto, Lucas Adolfo Mauro, Fernández, Pablo Marcelo, Castellanos de Figueroa, Lucía Inés, Mac Cormack, Walter Patricio
Format: Article
Language:English
Subjects:
Adaptation, Physiological
Antarctic Regions
antarctica
Ascomycota - enzymology
Ascomycota - isolation & purification
Ascomycota - metabolism
Basidiomycota - enzymology
Basidiomycota - isolation & purification
Basidiomycota - metabolism
Biodegradation, Environmental
Biomass
Bioprospecting
Biotechnology
Cold Temperature
DNA, Fungal
enzymes
Fungal Proteins - genetics
Gasoline
Hydrocarbons - metabolism
Phylogeny
Pichia - enzymology
Pichia - growth & development
Pichia - isolation & purification
Soil Microbiology
yeasts
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Summary:The aim of this study was to investigate the ability to produce extracellular hydrolytic enzymes at low temperature of yeasts isolated from 25 de Mayo island, Antarctica, and to identify those exhibiting one or more of the evaluated enzymatic activities. A total of 105 yeast isolates were obtained from different samples and 66 were identified. They belonged to 12 basidiomycetous and four ascomycetous genera. Most of the isolates were ascribed to the genera Cryptococcus, Mrakia, Cystobasidium, Rhodotorula, Gueomyces, Phenoliferia, Leucosporidium, and Pichia. Results from enzymes production at low temperatures revealed that the Antarctic environment contains metabolically diverse cultivable yeasts, which represent potential tools for biotechnological applications. While most the isolates proved to produce 2‐4 of the investigated exoenzymes, two of them evidenced the six evaluated enzymatic activities: Pichia caribbica and Guehomyces pullulans, which were characterized as psycrotolerant and psycrophilic, respectively. In addition, P. caribbica could assimilate several n‐alkanes and diesel fuel. The enzyme production profile and hydrocarbons assimilation capacity, combined with its high level of biomass production and the extended exponential growth phase make P. caribbica a promising tool for cold environments biotechnological purposes in the field of cold‐enzymes production and oil spills bioremediation as well.
ISSN:0233-111X
1521-4028
DOI:10.1002/jobm.201700021