The First Nucleotide of the Codon Located in the P Site of the 80S Ribosome Is Close to G1702 of the 18S rRNA as Revealed by Crosslinking to a pUUUGUU Derivative Containing a Perfluorophenylazido Group at the Guanine

Modification of the 18S rRNA with a pUUUGUU derivative carrying a perfluorophenylazido group at N7 of G was studied in the complex with the human 80S ribosome and Val-tRNA super(Val), which directs modified GUU to the P site. Reverse transcription reported modification of invariant G1702 of the 18S...

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Bibliographic Details
Published in:Molecular biology (New York) 2004-05, Vol.38 (3), p.421-426
Main Authors: Demeshkina, NA, Repkova, M N, Ven'yaminova, A G, Graifer, D M, Karpova, G G
Format: Article
Language:English
Subjects:
Codons
Data processing
Genes
Guanine
Microbiology
Nucleotides
Reverse transcription
Ribosomes
rRNA 18S
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Summary:Modification of the 18S rRNA with a pUUUGUU derivative carrying a perfluorophenylazido group at N7 of G was studied in the complex with the human 80S ribosome and Val-tRNA super(Val), which directs modified GUU to the P site. Reverse transcription reported modification of invariant G1702 of the 18S rRNA. On evidence of the results and the earlier data on affinity modification of the human ribosome with tetra- or heptaribonucleotide derivatives carrying an alkylating group at the 3' end, the template was assumed to make a bend between the A- and P-site codons, which brings both codons closer to G1702 of the 18S rRNA.
ISSN:0026-8933
1608-3245
DOI:10.1023/B:MBIL.0000032214.32927.8c