The effect of orexin 1 and orexin 2 receptors antagonisms in the basolateral amygdala on memory processing in a passive avoidance task

• Published in: Physiology & behavior 2017-05, Vol.174, p.42-48
• Main Authors: Ardeshiri, Motahareh Rouhi, Hosseinmardi, Narges, Akbari, Esmaeil
• Format: Article
• Language: English
• Subjects: Animals; Avoidance Learning - physiology; Basolateral amygdala; Basolateral Nuclear Complex - drug effects; Basolateral Nuclear Complex - physiology; Benzoxazoles - pharmacology; Dose-Response Relationship, Drug; Isoquinolines - pharmacology; Learning; Male; Memory; Memory - drug effects; Mental Recall - drug effects; Orexin Receptor Antagonists - pharmacology; Orexin receptors; Passive avoidance learning; Psychiatry; Pyridines - pharmacology; Rats; Rats, Wistar; Reaction Time - drug effects; Urea - analogs & derivatives; Urea - pharmacology
• ISSN: 0031-9384; 1873-507X
• DOI: 10.1016/j.physbeh.2017.03.004

Abstract There is an extensive evidence concerning basolateral amygdala (BLA) function to hippocampal memory processing. However, few researches have addressed the orexinergic system roles in this modulation. Then, the present study aims at investigating the action of orexin 1 and 2 receptors in BLA on passive avoidance (PA) learning. Wistar male rats (n = 79) were trained to avoid foot shock in one type of PA task. The rats were injected bilaterally into BLA, a selective orexin 1 receptor antagonist, SB-334867-A (3, 6, 12 μg/0.5 μl), and an orexin 2 receptor antagonist, TCS-OX2–29 (2.5, 5, 10 μg/0.5 μl), after training or before retrieval of the inhibitory avoidance task. Control rats received dimethyl sulfoxide at the same volume. The amount of learning was assessed 24 h later. The time of the first entrance to the dark compartment and the total time spending in the light compartment were measured as criteria for the avoidance memory. The results showed that consolidation and retrieval were significantly impaired by SB-334867-A administration into BLA in 3, 6 and 12 μg/0.5 μl. However, TCS-OX2-29 in 2.5 μg/0.5 μl could not influence neither consolidation nor retrieval. The TCS-OX2-29 administration into BLA impaired memory retrieval in 5 and 10 μg/0/5 μl but not the consolidation. It gives the impression that orexinergic system of the BLA plays an important role in regulation of memory processing and learning in the rats.