The Effects of Gold Nanoparticles Functionalized with ß -Amyloid Specific Peptides on an in vitro Model of Blood–Brain Barrier

Abstract We studied the effect of gold nanoparticle (AuNP) size, surface charge, concentration and morphology on the integrity of the Blood–Brain-Barrier (BBB) in a well-established in vitro model set-up. We focused on the effect of peptide functionalized hollow gold nanospheres and gold nanorods, w...

Full description

Saved in:
Bibliographic Details
Published in:Nanomedicine 2017-07, Vol.13 (5), p.1645-1652
Main Authors: Ruff, Julie, Hüwel, Sabine, Kogan, M.J, Simon, U, Galla, H.J
Format: Article
Language:English
Subjects:
Alzheimer Disease - drug therapy
Amyloid beta-Peptides
Biological Transport
Blood-Brain Barrier
Gold
Gold nanorods
Hollow gold nanospheres
Humans
Impedance spectroscopy
Internal Medicine
Metal Nanoparticles
Peptides
Protein Binding
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Abstract We studied the effect of gold nanoparticle (AuNP) size, surface charge, concentration and morphology on the integrity of the Blood–Brain-Barrier (BBB) in a well-established in vitro model set-up. We focused on the effect of peptide functionalized hollow gold nanospheres and gold nanorods, which selectively bind to amyloidogenic β -amyloid structures. These AuNP conjugates have already been successfully tested as photothermal absorbers for potential application in Alzheimer's disease (AD) therapy in an in vitro set-up, but may exhibit a low passage through the BBB due to their overall negative charge. Our results show that: (i) small (1.4 nm) AuNP strongly affect the BBB integrity, (ii) negative surface charge impedes BBB passage, and (iii) this charge effect caused by the peptide is compensated by covalent coupling to a polyethylene glycol ligand stabilizing the particles in diluted manner.
ISSN:1549-9634
1549-9642
DOI:10.1016/j.nano.2017.02.013