PRIMER NOTE: Characterization of 10 trinucleotide microsatellite loci in the Critically Endangered Pyrenean yam Borderea chouardii (Dioscoreaceae)

The low levels of allozymic variability found in the Critically Endangered Borderea chouardii prompted us to develop microsatellite markers to assess the genetic variability and population structure for the adequate conservation management of this species. A (CTT) sub(@)n-enriched partial genomic li...

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Bibliographic Details
Published in:Molecular ecology notes 2003-06, Vol.3 (2), p.265-267
Main Authors: Segarra-Moragues, J G, Palop-Esteban, M, Gonzalez-Candelas, F, Catalan, P
Format: Article
Language:English
Online Access:Get full text
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Summary:The low levels of allozymic variability found in the Critically Endangered Borderea chouardii prompted us to develop microsatellite markers to assess the genetic variability and population structure for the adequate conservation management of this species. A (CTT) sub(@)n-enriched partial genomic library was constructed. Ten polymorphic microsatellite loci were isolated from it, rendering 51 alleles in 47 individuals analysed. The allelic pattern observed for all of the loci with more than two alleles suggests that B. chouardii is tetraploid.
ISSN:1471-8278
DOI:10.1046/j.1471-8286.2003.00422.x