The pharmacodynamic active components study of Tibetan medicine Gentianopsis paludosa on ulcerative colitis fibrosis

• Published in: International immunopharmacology 2017-05, Vol.46, p.163-169
• Main Authors: Lu, Nian-hua, Zhao, Hui-qiao, Jing, Ming, Liu, Xiong, Ren, Chun-zhen, Liu, Xue-feng, Liu, Juan-juan, Zhang, Yan-xia
• Format: Article
• Language: English
• Subjects: Actin; Active component; Animals; Benzene; Benzenesulfonic acids; Cells, Cultured; China; Chromatography; Colitis, Ulcerative - chemically induced; Colitis, Ulcerative - drug therapy; Collagen; Collagen (type I); Collagen (type III); Colon; Colon - drug effects; Colon - pathology; Column chromatography; Diarrhea; E-cadherin; Ethanol; Ethanol - chemistry; Exploitation; Fibrosis; Flowers & plants; Gene expression; Gentianaceae - immunology; Gentianopsis; Gentianopsis paludosa; Herbal medicine; Humans; Inflammatory bowel disease; Inflammatory bowel diseases; Infrared spectroscopy; Intestine; Mass spectroscopy; Medicine, Tibetan Traditional; mRNA; Muscles; NMR; Nuclear magnetic resonance; Pharmacodynamics; Pharmacology; Plant Extracts - chemistry; Plant Extracts - pharmacology; Polarity; Rats; Rats, Wistar; Silica; Silica gel; Silicon dioxide; Smooth muscle; Sulfonic acid; Tibetan medicine; Tissues; Trinitrobenzenesulfonic Acid; Ulcerative colitis; Ulcerative colitis fibrosis; Ultraviolet spectroscopy; Xanthones - pharmacology
• ISSN: 1567-5769; 1878-1705
• DOI: 10.1016/j.intimp.2017.01.001

The current study focused on the pharmacodynamic activity components of Gentianopsis paludosa against ulcerative colitis (UC) fibrosis including symptoms of intestinal diarrhea and inflammatory. Trinitro-benzene-sulfonic acid induced UC model rats were gavaged with gradient polarity extracts respectively from ethanol-extract of Gentianopsis paludosa. Masson staining and qRT-PCR methods were respectively used to assess the degree of UC fibrosis and detect the mRNA expressions of collagen I, collagen III, a-smooth muscle actin (α-SMA) and E-cadherin in colon tissue. Separated by silica gel column chromatography, further screening was conducted until active components appeared. Infrared, nuclear magnetic resonance, mass spectroscopy and ultraviolet methods were applied to confirm active components' structures. The results indicated that the expression of collagen I, collagen III and α-SMA mRNA in the colon tissues of acetidin group rats was obviously depressed compared with control groups while E-cadherin displayed just opposite. Dyed in blue indicating UC fibrosis degree, the area of acetidin group was less than that other experimental groups. Four components: (1,8-Dihydroxy-3,7-Dimethoxyxanthones, 1-hydroxy-3,7,8-Trimethoxyxanthones, 1,7-Dihydroxy-3,8-Dimethoxyxanthones and 1-hydroxy-3,7-Dimethoxyxanthones), were obtained from acetidin group and all of which have a significant equivalence to Gentianopsis paludosa on the therapeutic effect of UC fibrosis. Our findings revealed the activity components for clinical application history of Gentianopsis paludosa and provided a preliminary foundation for further new drug research and exploitation. •Effective active ingredients of the Tibetan medicinal herbs (Gentianopsis paludosa) for the treatment of ulcerative colitis•Masson staining and qRT-RNA methods were used to evaluate the changes of colon fibrosis before and after the intervention.•The improvement of colonic fibrosis in experimental model rats relies heavily on four xanthone compounds.