Large‐scale image‐based screening and profiling of cellular phenotypes

Cellular phenotypes are observable characteristics of cells resulting from the interactions of intrinsic and extrinsic chemical or biochemical factors. Image‐based phenotypic screens under large numbers of basal or perturbed conditions can be used to study the influences of these factors on cellular...

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Bibliographic Details
Published in:Cytometry. Part A 2017-02, Vol.91 (2), p.115-125
Main Authors: Bougen‐Zhukov, Nicola, Loh, Sheng Yang, Lee, Hwee Kuan, Loo, Lit‐Hsin
Format: Article
Language:English
Subjects:
automated image analysis
Cell Tracking
cellular phenotypes
Computation
Computer applications
Cytometry
Data processing
High-Throughput Screening Assays - methods
high‐content screening
high‐throughput microscopy
Humans
Image Processing, Computer-Assisted - methods
imaging‐based phenotypic screens
Molecular Imaging - methods
Phenotype
phenotypic profiling
Representations
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Summary:Cellular phenotypes are observable characteristics of cells resulting from the interactions of intrinsic and extrinsic chemical or biochemical factors. Image‐based phenotypic screens under large numbers of basal or perturbed conditions can be used to study the influences of these factors on cellular phenotypes. Hundreds to thousands of phenotypic descriptors can also be quantified from the images of cells under each of these experimental conditions. Therefore, huge amounts of data can be generated, and the analysis of these data has become a major bottleneck in large‐scale phenotypic screens. Here, we review current experimental and computational methods for large‐scale image‐based phenotypic screens. Our focus is on phenotypic profiling, a computational procedure for constructing quantitative and compact representations of cellular phenotypes based on the images collected in these screens. © 2016 International Society for Advancement of Cytometry
ISSN:1552-4922
1552-4930
DOI:10.1002/cyto.a.22909