Transcriptome and proteome analyses reveal complex mechanisms of reproductive diapause in the two‐spotted spider mite, Tetranychus urticae

Although a variety of factors underlying diapause have been identified in arthropods and other organisms, the molecular mechanisms regulating diapause are still largely unknown. Here, to better understand this process, we examined diapause‐associated genes in the two‐spotted spider mite, Tetranychus...

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Bibliographic Details
Published in:Insect molecular biology 2017-04, Vol.26 (2), p.215-232
Main Authors: Zhao, J.‐Y., Zhao, X.‐T., Sun, J.‐T., Zou, L.‐F., Yang, S.‐X., Han, X., Zhu, W.‐C., Yin, Q., Hong, X.‐Y.
Format: Article
Language:English
Subjects:
Anaerobiosis
Animals
Araneae
Arthropoda
Ca2+ signalling
Calcium Signaling
Cytoskeleton - metabolism
diapause
Diapause, Insect
Female
Gene Expression Profiling
Neurotransmitter Agents - metabolism
Proteome
Reproduction
Sequence Analysis, RNA
Tetranychidae - physiology
Tetranychus urticae
Transcriptome
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Summary:Although a variety of factors underlying diapause have been identified in arthropods and other organisms, the molecular mechanisms regulating diapause are still largely unknown. Here, to better understand this process, we examined diapause‐associated genes in the two‐spotted spider mite, Tetranychus urticae, by comparing the transcriptomes and proteomes of early diapausing and reproductive adult females. Amongst genes underlying diapause revealed by the transcriptomic and proteomic data sets, we described the noticeable change in Ca2+‐associated genes, including 65 Ca2+‐binding protein genes and 23 Ca2+ transporter genes, indicating that Ca2+ signalling has a substantial role in diapause regulation. Other interesting changes in diapause included up‐regulation of (1) glutamate receptors that may be involved in synaptic plasticity changes, (2) genes involved in cytoskeletal reorganization including genes encoding each of the components of thick and thin filaments, tubulin and members of integrin signalling and (3) genes involved in anaerobic energy metabolism, which reflects a shift to anaerobic energy metabolism in early diapausing mites.
ISSN:0962-1075
1365-2583
DOI:10.1111/imb.12286