Synthesis of biologically active human interferon alpha -2b in Nicotiana benthamiana

A method was developed for the production and purification of biologically active recombinant human interferon alpha -2b (rhIFN alpha -2b) synthesized by expression in Nicotiana benthamiana plants. A gene construct containing a modified hIFN alpha -2b gene was cloned in two vectors based on tobacco...

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Bibliographic Details
Published in:Applied biochemistry and microbiology 2016-12, Vol.52 (7), p.705-713
Main Authors: Kosobokova, EN, Piniugina, M V, Kosorukov, V S
Format: Article
Language:English
Online Access:Get full text
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Summary:A method was developed for the production and purification of biologically active recombinant human interferon alpha -2b (rhIFN alpha -2b) synthesized by expression in Nicotiana benthamiana plants. A gene construct containing a modified hIFN alpha -2b gene was cloned in two vectors based on tobacco mosaic virus driven by an actin promoter from Arabidopsis thaliana (pA-IFN-A) and cauliflower mosaic virus driven by a 35S promoter (pA-IFN-S). The expression vectors were introduced into the plant cells by agroinfiltration. The maximum rates of synthesis achieved in the case of pA-IFN-A and pA-IFN-S 5 days after agroinfiltration were determined to be 200 and 20 mg per 1 kg of fresh leaves, respectively. The recombinant hIFN alpha -2b synthesized in the plant showed high antiviral and antitumor activity comparable with that of commercial drug.
ISSN:0003-6838
1608-3024
DOI:10.1134/S0003683816070048