Differential immune responses in mice infected with the tissue-dwelling nematode Trichinella zimbabwensis

To improve diagnostic tools, immunotherapies and vaccine development for trichinellosis surveillance and control there is a need to understand the host immune responses induced during infection with Trichinella zimbabwensis, a tissue-dwelling nematode. In this study, we sought to determine immune re...

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Bibliographic Details
Published in:Journal of helminthology 2016-09, Vol.90 (5), p.547-554
Main Authors: Onkoba, W.N., Chimbari, M.J., Kamau, J.M., Mukaratirwa, S.
Format: Article
Language:English
Subjects:
Adaptive Immunity
Alligators and Crocodiles - parasitology
Animals
Antibodies, Helminth - blood
Crocodylus niloticus
Cytokines - blood
Disease Models, Animal
Enzyme-Linked Immunosorbent Assay
Female
Immunity, Innate
Immunoglobulin G - blood
Immunoglobulin M - blood
Mice, Inbred BALB C
Nematoda
Research Papers
T-Lymphocytes, Regulatory - immunology
Th1 Cells - immunology
Th2 Cells - immunology
Trichinella
Trichinella - immunology
Trichinella - isolation & purification
Trichinellosis - immunology
Trichinellosis - parasitology
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Summary:To improve diagnostic tools, immunotherapies and vaccine development for trichinellosis surveillance and control there is a need to understand the host immune responses induced during infection with Trichinella zimbabwensis, a tissue-dwelling nematode. In this study, we sought to determine immune responses induced in mice during T. zimbabwensis infection. The parasite strain used (Code ISS1209) was derived from a naturally infected crocodile (Crocodylus niloticus) and is the main Trichinella species prevalent in southern Africa. Sixty 6- to 8-week-old female BALB/c mice were randomly assigned to two equal groups: T. zimbabwensis-infected (n= 30) and the non-infected control group (n= 30). Levels of serum tumour necrosis factor-alpha (TNF-α), interleukin-10 (IL-10), interleukin-4 (IL-4) as well as parasite-specific IgM, IgG, IgG1, IgG2a, IgG2b and IgG3 antibody responses were determined using enzyme-linked immunosorbent assay (ELISA). The cytokines and antibodies provided information on T-helper 1 (Th1)- and Th2-type, T-regulatory and antibody responses. Results showed that during the intestinal stage of infection, higher levels of parasite-specific IgM, IgG, IgG1 (P
ISSN:0022-149X
1475-2697
DOI:10.1017/S0022149X15000723