The role of neutrophil myeloperoxidase in the development of inflammation induced by thermal skin burns

• Published in: Biochemistry (Moscow). Supplement. Series B, Biomedical chemistry Biomedical chemistry, 2017, Vol.11 (1), p.72-75
• Main Authors: Mikhalchik, E. V., Budkevich, L. I., Piterskaya, Yu. A., Penkov, L. Yu, Astamirova, T. S., Smolina, N. V., Vakhrusheva, T. V., Panasenko, O. M.
• Format: Article
• Language: English
• Subjects: Biomedical research; Bioorganic Chemistry; Burns; Chemistry; Chemistry and Materials Science; Inflammation; Luminescence; Medicinal Chemistry; Neutrophils
• ISSN: 1990-7508; 1990-7516
• DOI: 10.1134/S1990750817010073

Luminol-dependent chemiluminescence (CL) of blood neutrophils stimulated with phorbol-12- myristate-13-acetate (PMA) and myeloperoxidase (MPO) activity of neutrophils and plasma have been investigated in children ( n = 16) during the early period (1−7 days) after thermal skin burns exceeding 20% of total body surface. The CL level of stimulated neutrophils was higher in burn patients than in healthy children of the reference group ( p lt; 0.01). Increased neutrophil MPO activity was found in 40% of patients, while increased plasma MPO activity was detected in 57% of patients. The albumin fraction isolated from plasma of burn patients increased the PMA-stimulated CL response of blood from healthy donors. These results suggest that the acute inflammatory response to the thermal burn causes neutrophil activation and MPO release into plasma. MPO-mediated modification of plasma proteins, particularly albumin, may stimulate neutrophil activation and provoke further inflammatory response of the body to the thermal injury.