Characterization of isolates of equine infectious anemia virus in Brazil

Equine infectious anemia is an important infectious disease that affects equids worldwide. Control of the disease is currently based on detection of anti-p26 EIAV by Agar Gel Immunodiffusion (AGID). In this work, 62 animals were examined by AGID and nested-PCR using primers for the gag gene. Fifty-t...

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Bibliographic Details
Published in:Archives of virology 2017-03, Vol.162 (3), p.873-877
Main Authors: Tigre, Dellane Martins, Brandão, Camila Fonseca Lopes, de Paula, Fabiana Lopes, Chinalia, Fabio Alexandre, Campos, Gubio Soares, Sardi, Silvia Ines
Format: Article
Language:English
Subjects:
Anemia
Animals
Antibiotics
Asymptomatic
Biomedical and Life Sciences
Biomedicine
Brazil
Brief Report
Disease
Equine Infectious Anemia - virology
Genomes
Glycoproteins
Horses
Infectious Anemia Virus, Equine - classification
Infectious Anemia Virus, Equine - genetics
Infectious Anemia Virus, Equine - isolation & purification
Infectious Diseases
Medical Microbiology
Phylogeny
Polymerase Chain Reaction
Retroviridae
Viral Core Proteins - genetics
Virology
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Summary:Equine infectious anemia is an important infectious disease that affects equids worldwide. Control of the disease is currently based on detection of anti-p26 EIAV by Agar Gel Immunodiffusion (AGID). In this work, 62 animals were examined by AGID and nested-PCR using primers for the gag gene. Fifty-three samples (85.5%) were positive by nested-PCR, whereas only 33 samples (53%) were positive for AGID. Fifteen amplicons obtained by nested-PCR were sequenced and the aligned results subjected to phylogenetic analysis. The analysis suggests that the Brazilian EIAV form a cluster with WSU5, EIAVUK and Wyoming strains from United States.
ISSN:0304-8608
1432-8798
DOI:10.1007/s00705-016-3172-5