Determining Optimal Fluorescent Agent Concentrations in Dental Adhesive Resins for Imaging the Tooth/Restoration Interface

Fluorescent dyes like Rhodamine B (RB) have been used to identify the spatial distribution of adhesive restorative materials in the tooth/restoration interface. Potential effects of the addition of RB to dental adhesives were addressed in the past, but no further information is available on how to d...

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Bibliographic Details
Published in:Microscopy and microanalysis 2017-02, Vol.23 (1), p.122-130
Main Authors: Bim Júnior, Odair, Cebim, Marco A., Atta, Maria T., Machado, Camila M., Francisconi-dos-Rios, Luciana F., Wang, Linda
Format: Article
Language:English
Subjects:
Adhesives
Biological Applications
Bond strength
Composite Resins - chemistry
Dental Bonding
Dental Materials - chemistry
Dental Restoration, Permanent
Dentin
Dentin - diagnostic imaging
Dentin-Bonding Agents - chemistry
Dye concentrations
Dyes
Ethanol
Fluorescent Dyes - chemistry
Humans
Investigations
Labeling
Lasers
Materials Testing
Microscopy, Confocal
Resin Cements - chemistry
Resins
Resins, Synthetic - chemistry
Rhodamines
Spatial distribution
Spectrum analysis
Staining and Labeling - methods
Surface Properties
Teeth
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Summary:Fluorescent dyes like Rhodamine B (RB) have been used to identify the spatial distribution of adhesive restorative materials in the tooth/restoration interface. Potential effects of the addition of RB to dental adhesives were addressed in the past, but no further information is available on how to determine suitable concentrations of RB in these bonding agents for imaging in the confocal laser scanning microscope. This study provides systematical strategies for adding RB to viscous dental adhesive resins, focusing on the determination of the lowest range of dye concentrations necessary to achieve an acceptable image of the dentin/adhesive interface. It was demonstrated that optimized images of the resin distribution in dentin can be produced with 0.1–0.02 mg/mL of RB in the (tested) adhesives. Our approaches took into account aspects related to the dye concentration, photophysical parameters in different host media, specimen composition and morphology to develop a rational use of the fluorescent agent with the resin-based materials. Information gained from this work can help optimize labeling methods using dispersions of low-molecular-weight dyes in different monomer blend systems.
ISSN:1431-9276
1435-8115
DOI:10.1017/S143192761601271X