Cytogeography of the subalpine marsh marigold polyploid complex (Caltha leptosepala s.l., Ranunculaceae)

• Published in: American journal of botany 2017-02, Vol.104 (2), p.271-285
• Main Authors: Wefferling, Keir M., Castro, Sílvia, Loureiro, João, Castro, Mariana, Tavares, Daniela, Hoot, Sara B.
• Format: Article
• Language: English
• Subjects: Botany; Caltha; Caltha leptosepala; Cascades; Chromosomes, Plant - genetics; contact zone; cryptic species; cytogeography; Flow cytometry; Genome Size - genetics; Genome, Plant - genetics; Genomes; Geography; Morphology; Pacific Northwest; Ploidies; polyploid; Polyploidy; Population Density; Population Dynamics; Ranunculaceae; Ranunculaceae - classification; Ranunculaceae - genetics; Ranunculaceae - growth & development; refugia; RESEARCH ARTICLE; Rocky Mountains; Species Specificity; Wetlands
• ISSN: 0002-9122; 1537-2197
• DOI: 10.3732/ajb.1600365

PREMISE OF THE STUDY: Unrecognized variation in ploidy level can lead to an underestimation of species richness and a misleading delineation of geographic range. Caltha leptosepala (Ranunculaceae) comprises a complex of hexaploids (6x), rare nonaploids (9x), and dodecaploids (12x), all with unknown distributions. We delineate the geographic distribution and contact zones of the cytotypes, investigate morphologies of cytotypes and subspecies, and discuss the biogeography and evolutionary history of the polyploid complex. METHODS: Using cytologically determined specimens as reference, propidium iodide flow cytometry was performed on silica‐dried samples and herbarium specimens from across the range of C. leptosepala s.l. Genome size estimates from flow cytometry were used to infer cytotypes. A key morphological character, leaf length‐to‐width ratio, was measured to evaluate whether these dimensions are informative for taxon and/or cytotype delimitation. KEY RESULTS: Dodecaploids were more northerly in distribution than hexaploids, and a single midlatitude population in the Northern Rockies yielded nonaploids. Genome size estimates were significantly different between all cytotypes and between hexaploid subspecies. Leaf length‐to‐width ratios were significantly different between subspecies and some cytotypes. CONCLUSIONS: Caltha leptosepala presents clear patterns of cytotype distribution at the large scale. Marked differences in morphology, range, and genome size were detected between the hexaploid subspecies, C. leptosepala subsp. howellii in the Cascade‐Sierra axis and C. leptosepala subsp. leptosepala in the Rockies. Sympatry between cytotypes in the Cascades and a parapatric distribution in the Northern Rockies suggest unique origins and separate lineages in the respective contact zones.