Jasmonic acid-mediated defense suppresses brassinosteroid-mediated susceptibility to Rice black streaked dwarf virus infection in rice

• Published in: The New phytologist 2017-04, Vol.214 (1), p.388-399
• Main Authors: He, Yuqing, Zhang, Hehong, Sun, Zongtao, Li, Junmin, Hong, Gaojie, Zhu, Qisong, Zhou, Xuebiao, MacFarlane, Stuart, Yan, Fei, Chen, Jianping
• Format: Article
• Language: English
• Subjects: Acetates - pharmacology; brassinosteroid pathway; Brassinosteroids - pharmacology; Cyclopentanes - pharmacology; Disease Susceptibility; Gene Expression Regulation, Plant - drug effects; Genes, Plant; jasmonic acid pathway; Oryza - drug effects; Oryza - genetics; Oryza - virology; Oryza sativa (rice); Oxylipins - pharmacology; plant defense response; Plant Diseases - virology; Plant Immunity - drug effects; Plant Leaves - drug effects; Plant Leaves - virology; Plant Proteins - genetics; Plant Proteins - metabolism; Plant Viruses - drug effects; Plant Viruses - physiology; Reoviridae; Rice Black Streaked Dwarf Virus (RBSDV); Signal Transduction - drug effects; Stress, Physiological - drug effects; Stress, Physiological - genetics; Transcription, Genetic - drug effects
• ISSN: 0028-646X; 1469-8137
• DOI: 10.1111/nph.14376

Plant hormones play a vital role in plant immune responses. However, in contrast to the relative wealth of information on hormone-mediated immunity in dicot plants, little information is available on monocot–virus defense systems. We used a high-throughput-sequencing approach to compare the global gene expression of Rice black-streaked dwarf virus (RBSDV)-infected rice plants with that of healthy plants. Exogenous hormone applications and transgenic rice were used to test RBSDV infectivity and pathogenicity. Our results revealed that the jasmonic acid (JA) pathway was induced while the brassinosteroid (BR) pathway was suppressed in infected plants. Foliar application of methyl jasmonate (MeJA) or brassinazole (BRZ) resulted in a significant reduction in RBSDV incidence, while epibrassinolide (BL) treatment increased RBSDV infection. Infection studies using coi1-13 and Go mutants demonstrated JA-mediated resistance and BR-mediated susceptibility to RBSDV infection. A mixture of MeJA and BL treatment resulted in a significant reduction in RBSDV infection compared with a single BL treatment. MeJA application efficiently suppressed the expression of BR pathway genes, and this inhibition depended on the JA coreceptor OsCOI1. Collectively, our results reveal that JA-mediated defense can suppress the BR-mediated susceptibility to RBSDV infection.