Influence of dexamethasone-induced stress on oxidative stress biomarkers in non-pregnant does experimentally infected with Brucella melitensis

Oxidative stress and its biomarkers are considered emerging fields in ruminant medicine. Data on this emerging field in dexamethasone-treated goats infected with Brucella melitensis is limited. This study aimed at evaluating biomarkers of oxidative stress in dexamethasone-treated non-pregnant Boer g...

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Bibliographic Details
Published in:Comparative clinical pathology 2017-03, Vol.26 (2), p.423-435
Main Authors: Polycarp, Tanko N., M. Yusoff, Sabri, Benjamin, Emikpe O., M. Salisi, Shahrom, Bejo, Siti-Khairani
Format: Article
Language:English
Subjects:
Brucella melitensis
Hematology
Medicine
Medicine & Public Health
Oncology
Original Article
Pathology
Ruminantia
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Summary:Oxidative stress and its biomarkers are considered emerging fields in ruminant medicine. Data on this emerging field in dexamethasone-treated goats infected with Brucella melitensis is limited. This study aimed at evaluating biomarkers of oxidative stress in dexamethasone-treated non-pregnant Boer goats experimentally infected with B. melitensis . Eighteen healthy, non-pregnant goats were divided into three groups: A, B, and C of six animals each. Group A was treated with dexamethasone (2 mg/kg) for 7 days prior to inoculation with 10 7  CFU of B. melitensis ocularly. Group B was not treated but inoculated with similar dose of B. melitensis as in group A above while group C was administered normal saline. Blood samples were collected periodically through day 42 post inoculation (pi). Plasma cortisol, malondialdehyde (MDA), xanthine oxidase (XO), superoxide dismutase (SOD), glutathione (GSH), and serological responses (independent enzyme-linked immunosorbent assay and Rose Bengal Plate Test) were analyzed. Three animals from each group were sacrificed at days 21 and 42 pi and samples from the liver, kidney, and lungs were collected and processed for further analysis. Superoxide dismutase, GSH, and XO activities increased significantly ( P  
ISSN:1618-5641
1618-565X
DOI:10.1007/s00580-016-2395-x