Repair of segmental radial defects in dogs using tailor-made titanium mesh cages with plates combined with calcium phosphate granules and basic fibroblast growth factor-binding ion complex gel

• Published in: Journal of artificial organs 2017-03, Vol.20 (1), p.91-98
• Main Authors: Honnami, Muneki, Choi, Sungjin, Liu, I-li, Kamimura, Wataru, Taguchi, Tetsushi, Ichimura, Makoto, Urushisaki, Yukinori, Hojo, Hironori, Shimohata, Nobuyuki, Ohba, Shinsuke, Amaya, Koichi, Koyama, Hiroyuki, Nishimura, Ryohei, Chung, Ung-il, Sasaki, Nobuo, Mochizuki, Manabu
• Format: Article
• Language: English
• Subjects: Animals; Biomedical Engineering and Bioengineering; Bone Plates; Bone Regeneration - drug effects; Bone Regeneration - physiology; Calcium Phosphates - therapeutic use; Cardiac Surgery; Dogs; Fibroblast Growth Factor 2 - therapeutic use; Medicine; Medicine & Public Health; Nephrology; Original Article; Radius - surgery; Titanium; Weight-Bearing; Wound Healing - drug effects; Wound Healing - physiology
• ISSN: 1434-7229; 1619-0904
• DOI: 10.1007/s10047-016-0918-5

Repair of large segmental defects of long bones are a tremendous challenge that calls for a novel approach to supporting immediate weight bearing and bone regeneration. This study investigated the functional and biological characteristics of a combination of a tailor-made titanium mesh cage with a plate (tTMCP) with tetrapod-shaped alpha tricalcium phosphate granules (TB) and basic fibroblast growth factor (bFGF)-binding ion complex gel (f-IC gel) to repair 20-mm segmental radial defects in dogs. The defects were created surgically in 18 adult beagle dogs and treated by implantation of tTMCPs with TB with (TB-gel group) or without (TB group) f-IC gel. Each tTMCP fitted the defect well, and all dogs could bear weight on the affected limb immediately after surgery. Dogs were euthanized 4, 8 and 24 weeks after implantation. Histomorphometry showed greater infiltration of new vessels and higher bone union rate in the TB-gel group than in the TB group. The lamellar bone volume and mineral apposition rate did not differ significantly between the groups, indicating that neovascularization may be the primary effect of f-IC gel on bone regeneration. This combination method which is tTMCP combined with TB and f-IC gel, would be useful for the treatment of segmental long bone defects.