Pramipexole, a Dopamine D2/D3 Receptor-Preferring Agonist, Prevents Experimental Autoimmune Encephalomyelitis Development in Mice

• Published in: Molecular neurobiology 2017-03, Vol.54 (2), p.1033-1045
• Main Authors: Lieberknecht, Vicente, Junqueira, Stella C., Cunha, Mauricio P., Barbosa, Thaís A., de Souza, Luiz F., Coelho, Igor S., Santos, Adair R. S., Rodrigues, Ana Lúcia S., Dafré, Alcir L., Dutra, Rafael C.
• Format: Article
• Language: English
• Subjects: Animals; Autoimmune diseases; Benzothiazoles - pharmacology; Benzothiazoles - therapeutic use; Biomedical and Life Sciences; Biomedicine; Cell Biology; Dopamine; Dopamine Agonists - pharmacology; Dopamine Agonists - therapeutic use; Encephalomyelitis, Autoimmune, Experimental - immunology; Encephalomyelitis, Autoimmune, Experimental - pathology; Encephalomyelitis, Autoimmune, Experimental - prevention & control; Female; Mice; Mice, Inbred C57BL; Multiple sclerosis; Neurobiology; Neurology; Neurosciences; Receptors, Dopamine D2 - agonists; Receptors, Dopamine D2 - immunology; Receptors, Dopamine D3 - agonists; Receptors, Dopamine D3 - immunology
• ISSN: 0893-7648; 1559-1182
• DOI: 10.1007/s12035-016-9717-5

Experimental autoimmune encephalomyelitis (EAE) is the most used animal model of multiple sclerosis (MS) for the development of new therapies. Dopamine receptors can modulate EAE and MS development, thus highlighting the potential use of dopaminergic agonists in the treatment of MS, which has been poorly explored. Herein, we hypothesized that pramipexole (PPX), a dopamine D2/D3 receptor-preferring agonist commonly used to treat Parkinson’s disease (PD), would be a suitable therapeutic drug for EAE. Thus, we report the effects and the underlying mechanisms of action of PPX in the prevention of EAE. PPX (0.1 and 1 mg/kg) was administered intraperitoneally (i.p.) from day 0 to 40 post-immunization (p.i.). Our results showed that PPX 1 mg/kg prevented EAE development, abolishing EAE signs by blocking neuroinflammatory response, demyelination, and astroglial activation in spinal cord. Moreover, PPX inhibited the production of inflammatory cytokines, such as IL-17, IL-1β, and TNF-α in peripheral lymphoid tissue. PPX was also able to restore basal levels of a number of EAE-induced effects in spinal cord and striatum, such as reactive oxygen species, glutathione peroxidase, parkin, and α-synuclein (α-syn). Thus, our findings highlight the usefulness of PPX in preventing EAE-induced motor symptoms, possibly by modulating immune cell responses, such as those found in MS and other T helper cell-mediated inflammatory diseases.