miRNA and piRNA expression profiles of breeder cock testes detected by next‐generation sequencing

Contents miRNAs are small non‐coding regulatory RNAs that play key roles in diverse biological processes. In this study, we used the Solexa sequencing technique to profile miRNAs in breeder cock testes to illustrate their functions. A total of 663 co‐expressed miRNAs and 3,180 co‐expressed piRNAs we...

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Bibliographic Details
Published in:Reproduction in domestic animals 2017-04, Vol.52 (2), p.203-213
Main Authors: Wu, SR, Guo, W, Li, YL, Ren, XC, Lei, XY, Li, XY, Yao, JH, Yang, XJ
Format: Article
Language:English
Subjects:
Animal reproduction
Animals
Biological activity
Chickens - physiology
Encyclopedias
Gene expression
Gene Expression Regulation - physiology
Genes
Genomes
Male
Metabolism
MicroRNAs - genetics
MicroRNAs - metabolism
miRNA
Nucleic Acid Amplification Techniques - methods
Nucleic Acid Amplification Techniques - veterinary
Poultry
Ribonucleic acid
RNA
Spermatogenesis
Spermatozoa
Testes
Testis - metabolism
Transcriptome - physiology
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Summary:Contents miRNAs are small non‐coding regulatory RNAs that play key roles in diverse biological processes. In this study, we used the Solexa sequencing technique to profile miRNAs in breeder cock testes to illustrate their functions. A total of 663 co‐expressed miRNAs and 3,180 co‐expressed piRNAs were detected in three libraries. Based on Mir‐X™ miRNA qRT‐PCR, three miRNAs representing low, medium and high expression levels according to the sequencing results were selected randomly to validate the miRNAs' expression profiles. Results suggested that the miRNA expression profiles data could represent actual miRNA expression levels. Moreover, target genes prediction of the co‐expressed miRNAs and further Gene Ontology and Kyoto Encyclopedia of Genes and Genomes analyses were performed, which revealed that some candidate miRNAs were involved in the regulation of the spermatogenesis process, spermatozoa function and testicular metabolism. In conclusion, we provided a useful resource for further elucidation of the miRNAs' regulatory role in spermatogenesis, contributing to a preliminary database for functional and molecular mechanistic studies in testicular metabolism, spermatogenesis and other testes functions.
ISSN:0936-6768
1439-0531
DOI:10.1111/rda.12880