Latent murine leukemia virus infection characterized by the release of non-infectious virions

Abstract Clonal cell lines derived from cultures infected with a polytropic MuLV release vastly different levels of infectious virions ranging from undetectable to very high. Low producing clones release an overwhelming proportion of non-infectious virions containing retroviral RNA but deficient in...

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Bibliographic Details
Published in:Virology (New York, N.Y.) N.Y.), 2017-06, Vol.506, p.19-27
Main Authors: Boi, Stefano, Dis, Erik Van, Hansen, Ethan J, Rosenke, Kyle, Peterson, Karin E, Ferrell, Morgan E, Evans, Leonard H
Format: Article
Language:English
Subjects:
Animals
Gene Products, env - genetics
Gene Products, env - metabolism
Humans
Infectious Disease
Latency
Leukemia Virus, Murine - genetics
Leukemia Virus, Murine - physiology
Mice
MuLV
Replication
Retroviridae Infections - virology
Retroviruses
Virion - genetics
Virion - physiology
Virions
Virus Latency
Virus Release
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Summary:Abstract Clonal cell lines derived from cultures infected with a polytropic MuLV release vastly different levels of infectious virions ranging from undetectable to very high. Low producing clones release an overwhelming proportion of non-infectious virions containing retroviral RNA but deficient in the Env protein. Non-infectious virion production is not due to an inability of the cells to support infectious MuLV production or to an inherent replicative defectiveness of the proviruses. Reinfection of the lowest producing lines with the polytropic or an ecotropic MuLV results in enormous increases in the specific infectivity of the released virions. This indicates a reversible state of retroviral latency characterized by the release of non-infectious virions that is likely the result of insufficient levels of Env protein required for infectivity. The latency state described here may have important roles in in vivo retroviral infections including alterations of the immune response and the production of defective interfering particles.
ISSN:0042-6822
1096-0341
DOI:10.1016/j.virol.2017.03.004