Comparison of the effects of Ham'sF10 and αMEM in combination with FBS or BSA in vitrification/warming solution on quality and viability of sheep ovarian follicles

Contents The aim of this study was to evaluate the effects of the two types of media, namely minimum essential medium (αMEM) and Ham'sF10, supplemented with foetal bovine serum (FBS) or bovine serum albumin (BSA) in vitrification/warming solution on the quality and viability of sheep ovarian fo...

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Bibliographic Details
Published in:Reproduction in domestic animals 2017-08, Vol.52 (4), p.551-560
Main Authors: Mohammadzadeh, F, Safdarian, L, Amidi, F, Mohammadzadeh, A, Mortezaee, K, Mehdinejhadiani, S, Sobhani, A, Ghasemi, S, Sargolzaei Aval, F
Format: Article
Language:English
Subjects:
Animals
bovine serum albumin
Cattle
Cryopreservation - methods
Cryopreservation - veterinary
Cryoprotective Agents
DNA Fragmentation
Female
Fetal Blood
Isotonic Solutions
Organic Chemicals
ovarian follicle
Ovarian Follicle - physiology
Serum Albumin, Bovine
Sheep
Tissue Culture Techniques - veterinary
Vitrification
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Summary:Contents The aim of this study was to evaluate the effects of the two types of media, namely minimum essential medium (αMEM) and Ham'sF10, supplemented with foetal bovine serum (FBS) or bovine serum albumin (BSA) in vitrification/warming solution on the quality and viability of sheep ovarian follicles. Vitrification method was applied for cryopreservation of sheep ovarian cortex using Ham'sF10 and αMEM supplemented with either BSA or FBS. There were five groups: Fresh, Ham'sF10+ BSA, Ham'sF10+ FBS, αMEM + BSA and αMEM + FBS. Samples were cultured for two weeks after warming. Viability and morphology of follicles and DNA fragmentation in follicles and in tissue stroma cells were analysed before vitrification/warming and following one and two weeks of culture. The Ham'sF10+ FBS and Ham'sF10+ BSA groups showed a significant decrease in follicular viability after one week of culture (p 
ISSN:0936-6768
1439-0531
DOI:10.1111/rda.12945