Epidermal powder immunization of mice and monkeys with an influenza vaccine

Epidermal powder immunization (EPI) with an influenza vaccine and an adjuvant such as QS-21, LTR72, or cholera toxin elicited augmented serum and mucosal antibody responses in mice. Rhesus macaques, which have an immune system and skin structure similar to humans, were used to further evaluate the i...

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Bibliographic Details
Published in:Vaccine 2003-06, Vol.21 (21), p.2830-2836
Main Authors: Chen, Dexiang, Endres, Ryan, Maa, Yuh-Fun, Kensil, Charlotte R., Whitaker-Dowling, Patricia, Trichel, Anita, Youngner, Julius S., Payne, Lendon G.
Format: Article
Language:English
Subjects:
Adjuvant
Adjuvants, Immunologic - administration & dosage
Administration, Cutaneous
Animals
Antibodies, Viral - blood
Biological and medical sciences
Cholera
Cholera Toxin - immunology
Enzyme-Linked Immunosorbent Assay
Female
Fundamental and applied biological sciences. Psychology
Hemagglutination Inhibition Tests
Immune system
Immunity, Mucosal
Immunization
Immunogenicity
Influenza
Influenza Vaccines - administration & dosage
Influenza Vaccines - immunology
Injection
Injections, Intramuscular
Macaca mulatta
Methods
Mice
Mice, Inbred BALB C
Microbiology
Monkeys & apes
Mortality
Mucosal antibody
Orthomyxoviridae Infections - immunology
Orthomyxoviridae Infections - prevention & control
Powders
Primate
Rodents
Saponins - administration & dosage
Saponins - immunology
Species Specificity
Toxins
Vaccination - methods
Vaccine
Vaccines
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies
Virology
Viruses
Waterborne diseases
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Description
Summary:Epidermal powder immunization (EPI) with an influenza vaccine and an adjuvant such as QS-21, LTR72, or cholera toxin elicited augmented serum and mucosal antibody responses in mice. Rhesus macaques, which have an immune system and skin structure similar to humans, were used to further evaluate the immunogenicity of the influenza vaccine following EPI. EPI of rhesus macaques with an influenza vaccine and QS-21 adjuvant elicited significantly higher serum hemagglutination inhibition (HI) titers than antigen alone administered by EPI or by intramuscular (IM) injection using a needle and syringe. In the absence of QS-21, EPI and IM injection elicited comparable HI titers in the monkeys. This study suggests that EPI is a promising technique for administering human vaccine and that QS-21 augments the immunogenicity of co-administered influenza vaccine.
ISSN:0264-410X
1873-2518
DOI:10.1016/S0264-410X(03)00175-0