Monitoring biofilm formation by using cyclic voltammetry-effect of the experimental conditions on biofilm removal and activity

The effect of experimental conditions on cyclic voltammetry experiments on platinum electrodes covered with biofilms formed by Pseudomonas fluorescens for 2 hours was investigated. Results show that recycling the potential stabilizes the shape of the cyclic voltammogram after 195 cycles, but the obs...

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Bibliographic Details
Published in:Water science and technology 2003-01, Vol.47 (5), p.51-56
Main Authors: Gião, M S, Montenegro, M I, Vieira, M J
Format: Article
Language:English
Subjects:
Bacteria
Bacteria (Pseudomonadaceae) (Pseudomonas)
Biofilms
Electrochemistry
Electrodes
Environmental Monitoring - methods
Microscopy
Microscopy - methods
Platinum
Pseudomonas
Pseudomonas fluorescens
Removal
Voltammetry
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Summary:The effect of experimental conditions on cyclic voltammetry experiments on platinum electrodes covered with biofilms formed by Pseudomonas fluorescens for 2 hours was investigated. Results show that recycling the potential stabilizes the shape of the cyclic voltammogram after 195 cycles, but the observation of the electrodes by epifluorescence microscopy showed that cells are still adhered to the platinum surface. Some experimental conditions were changed during the electrochemical measurements--sweep rate, pH of the buffer and applied potential range. Some of these parameters had a strong impact on the bacteria that are adhered to the surface, increasing the death and removal in some circumstances.
ISSN:0273-1223
1996-9732
DOI:10.2166/wst.2003.0278