method to determine tannin concentration by the measurement and quantification of protein-tannin interactions

Protein determinations on protein-tannin complexes after protein isolation (gel filtration and trichloroacetic acid [TCA] precipitation) or phenolic extraction (polyvinyl pyrrolidone [PVP] and organic solvent precipitation) were unsuccessful. Kjeldahl determinations of the amount of unprecipitated p...

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Bibliographic Details
Published in:Oecologia 1987-01, Vol.73 (3), p.420-424
Main Authors: Amory, A.M, Schubert, C.L
Format: Article
Language:English
Subjects:
Acacia
Alcohols
Animal and plant ecology
Animal, plant and microbial ecology
Biological and medical sciences
Chemical precipitation
Fundamental and applied biological sciences. Psychology
Gels
General aspects
Herbivores
Pisum sativum
Plant dietary protein
Precipitates
protein analysis
Quantification
Tannins
Viscosity
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Summary:Protein determinations on protein-tannin complexes after protein isolation (gel filtration and trichloroacetic acid [TCA] precipitation) or phenolic extraction (polyvinyl pyrrolidone [PVP] and organic solvent precipitation) were unsuccessful. Kjeldahl determinations of the amount of unprecipitated protein bovine serum albumin [BSA] showed a sigmoid relationship with increasing concentrations of tannins. A similar relationship was found for the reduced viscosity of BSA and plant protein, and the concentration of tannin. Non-linear regression and curve normalization allowed three variable (k1,k2and T1/2) to be defined for the quantification of the protein-tannin interaction/s. Such a treatment may be useful in studies of the role of tannins in plant-herbivore interactions.
ISSN:0029-8549
1432-1939
DOI:10.1007/BF00385259