Effect of green tea (Camellia sinensis) extract and pre-freezing equilibration time on the post-thawing quality of ram semen cryopreserved in a soybean lecithin-based extender

The aim of this study was to determine the effect of Camellia sinensis extract as antioxidant supplement and pre-freezing equilibration times in a soybean lecithin extender for freezing ram semen. In this study, a total of 20 ejaculates were collected from four Ghezel rams and diluted with extenders...

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Bibliographic Details
Published in:Cryobiology 2016-12, Vol.73 (3), p.297-303
Main Authors: Mehdipour, Mahdieh, Daghigh Kia, Hossein, Najafi, Abouzar, Vaseghi Dodaran, Hossein, García-Álvarez, Olga
Format: Article
Language:English
Subjects:
Camellia sinensis
Camellia sinensis extract
Cryopreservation
Equilibration time
Soybean lecithin
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Summary:The aim of this study was to determine the effect of Camellia sinensis extract as antioxidant supplement and pre-freezing equilibration times in a soybean lecithin extender for freezing ram semen. In this study, a total of 20 ejaculates were collected from four Ghezel rams and diluted with extenders (1.5% soybean lecithin, 7% glycerol) containing no supplements (control) and Camellia sinensis extract (5, 10, and 15 mg/L) and cryopreserved, immediately after thermal equilibrium was reached at 5 °C (0 h), or 4 h after equilibration. Sperm motility characteristics, membrane integrity, abnormal morphology, mitochondria activity, apoptotic status, MDA and antioxidant activities (GPx, SOD and total antioxidant capacity (TAC)) were evaluated following freeze-thawing. Camellia sinensis extract at level 10 mg/L led to the highest total and progressive motilities percentages, in comparison to other treatments (P 
ISSN:0011-2240
1090-2392
DOI:10.1016/j.cryobiol.2016.10.008