Isozyme analysis of relationships among Boesenbergia (Zingiberaceae) and related genera in Southern Thailand

Isozyme electrophoresis data of leaf extracts from 11 taxa of Boesenbergia, six taxa of Kaempferia and two taxa of Scaphochlamys from Southern Thailand were investigated. The nine enzyme systems used were peroxidase, superoxide dismutase, glutamate dehydrogenase, malate dehydrogenase, shikimate dehy...

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Bibliographic Details
Published in:Biochemical systematics and ecology 2003-05, Vol.31 (5), p.499-511
Main Authors: Vanijajiva, Ongkarn, Suvachittanont, Wallie, Sirirugsa, Puangpen
Format: Article
Language:English
Subjects:
Boesenbergia
Genetic relationships
Isozyme
Zingiberaceae
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Summary:Isozyme electrophoresis data of leaf extracts from 11 taxa of Boesenbergia, six taxa of Kaempferia and two taxa of Scaphochlamys from Southern Thailand were investigated. The nine enzyme systems used were peroxidase, superoxide dismutase, glutamate dehydrogenase, malate dehydrogenase, shikimate dehydrogenase, β-esterase, α-esterase, acid phosphatase and alkaline phosphatase. The first four were found to be useful as molecular markers for characterization of Boesenbergia and related taxa. Data obtained from the isozyme patterns from the samples were analyzed for the Nei and Li Dice similarity coefficient for pairwise comparison between individual samples and the distance matrix. The dendrogram resulting from cluster analysis and UPGMA shows a higher degree of relationship between Boesenbergia and Scaphochlamys than between Boesenbergia and Kaempferia. These findings were supported by a principal component analysis.
ISSN:0305-1978
1873-2925
DOI:10.1016/S0305-1978(02)00185-0