Determination of Triazines and Triazoles in Grapes Using Atmospheric Pressure Matrix-Assisted Laser Desorption/Ionization High-Resolution Mass Spectrometry

A chromatography-free atmospheric pressure matrix-assisted laser desorption/ionization high-resolution mass spectrometry (AP-MALDI HRMS) method is described for the simultaneous and quantitative detection of triazines and triazoles in grapes. The analytes were detected reproducibly with high mass ac...

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Bibliographic Details
Published in:Journal of AOAC International 2017-05, Vol.100 (3), p.640-646
Main Authors: Mahale, Vishal, Singh, Ajeet, Phadke, Gayatri S, Ghanate, Avinash D, Oulkar, Dasharath P, Banerjee, Kaushik, Panchagnula, Venkateswarlu
Format: Article
Language:English
Subjects:
Atmospheric Pressure
Chemical properties
Contamination
Grapes
Identification and classification
Mass Spectrometry
Methods
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Triazines - analysis
Triazoles
Triazoles - analysis
Vitis - chemistry
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Summary:A chromatography-free atmospheric pressure matrix-assisted laser desorption/ionization high-resolution mass spectrometry (AP-MALDI HRMS) method is described for the simultaneous and quantitative detection of triazines and triazoles in grapes. The analytes were detected reproducibly with high mass accuracy (mass error within 5 ppm) and further confirmed by collision-induced dissociation fragmentation in tandem MS. The LODs and LOQs for all the analytes were found to be in the nanogram per gram level (15-20 ng/g LOQ). Internal standard-normalized high-resolution accurate mass-extracted (HR-AM) peak intensities of the detected ions were used to generate the concentration response curves. Linearity (with R2 values around 0.99) was obtained for these curves within a concentration range of 20-200 ng/g of the individual analytes. The accuracy and precision of the method were further established using QC samples. Validation and performance comparison of the AP-MALDI HRMS method with an existing standard method using LC with triple quadrupole MS was carried out (evaluating sensitivity, accuracy, precision, and analysis time) using 20 table-grape field samples after QuEChERS extraction.
ISSN:1060-3271
1944-7922
DOI:10.5740/jaoacint.17-0047