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Oil-based adjuvants delivered intradermally induce high primary IgG2 immune response in swine

The effects of intradermal application of antigen with or without different adjuvants and activation of immune response are presented in this study. Six groups of six piglets each were immunized with keyhole limpet haemocyanin (KLH) antigen in combination with aluminium hydroxide or oil-based adjuva...

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Bibliographic Details
Published in:Research in veterinary science 2017-10, Vol.114, p.41-43
Main Authors: Oreskovic, Zrinka, Kudlackova, Hana, Krejci, Josef, Nechvatalova, Katerina, Faldyna, Martin
Format: Article
Language:English
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Summary:The effects of intradermal application of antigen with or without different adjuvants and activation of immune response are presented in this study. Six groups of six piglets each were immunized with keyhole limpet haemocyanin (KLH) antigen in combination with aluminium hydroxide or oil-based adjuvants (complete and incomplete Freund's adjuvants, Montanide ISA 206 and Emulsigen). IgG1 and IgG2 levels in sera were measured by KLH-specific ELISA. Interestingly, oil-based adjuvants induced high primary IgG2 response, suggesting the Th1 lymphocyte polarization. Also, considering the similarities between human and porcine organism, we suggest that intradermal application could be considered as an effective vaccine delivery route in both veterinary and human medicine. •Although intradermal application has many advantages, it is still seldom used.•Adjuvants are used in vaccine to influence quantity and quality of immune response.•Our results show that oil-based adjuvants enhances Th1 response with IgG2 isotype.
ISSN:0034-5288
1532-2661
DOI:10.1016/j.rvsc.2017.03.007