Development of 3‐D Intramural and Surface Potentials in the LV: Microstructural Basis of Preferential Transmural Conduction

• Published in: Journal of cardiovascular electrophysiology 2017-06, Vol.28 (6), p.692-701
• Main Authors: CALDWELL, BRYAN J., TREW, MARK L., LEGRICE, IAN J., SMAILL, BRUCE H.
• Format: Article
• Language: English
• Subjects: Action Potentials; Activation; Animal models; Animals; Anisotropy; Axes (reference lines); Cardiac Pacing, Artificial; computer modeling; Computer Simulation; Conduction; Density; Depolarization; electrical mapping; Electrical resistivity; Epicardial Mapping; extracellular potential; Heart; Heart Conduction System - anatomy & histology; Heart Conduction System - physiology; Heart diseases; Heart Rate; Heart Ventricles - anatomy & histology; Laminar; Mathematical models; Microstructure; Models, Animal; Models, Cardiovascular; Muscles; Recording; Stimulation; Surface chemistry; Sus scrofa; Time Factors; Ventricular Function, Left; ventricular microstructure; Xenografts
• ISSN: 1045-3873; 1540-8167
• DOI: 10.1111/jce.13207

Orthotropic Intramural Spread of LV Electrical Potentials Introduction Extracellular potentials measured on the heart surfaces are used to infer events that originate deep within the heart wall. We have reconstructed intramural potentials in three dimensions for the first time, and compare these with epicardial and endocardial surface potentials and cardiac microstructure. Methods and Results Extracellular potentials from intramural point stimulation were measured from a high density 3‐D electrode array in the in vivo pig LV. MR and extended volume imaging were used to register electrode locations and characterize fiber and laminar orientations throughout the recording volume. Measured potentials were compared with predictions of tissue‐specific bidomain computer activation models. Positive potentials recorded in the LV wall preceded the depolarization wavefront as it spread in the fiber direction. Transverse to this, passive and active potentials spread preferentially in the laminar direction (anisotropy ratio ∼1.6:1). Epicardial surface potentials reflect initial intramural propagation at the stimulus location, but endocardial potentials do not, particularly adjacent to papillary muscles. Measured 3‐D potentials were consistently better captured by computer models that incorporate three distinct conductivities aligned with local microstructural axes, but the preferential spread of potentials in the laminar direction was not fully predicted. Conclusions This study provides evidence for preferential transmural conduction and raises questions about the extent to which intramural electrical events can be inferred from endocardial potentials.