Smartphone based hand-held quantitative phase microscope using the transport of intensity equation method

In order to realize high contrast imaging with portable devices for potential mobile healthcare, we demonstrate a hand-held smartphone based quantitative phase microscope using the transport of intensity equation method. With a cost-effective illumination source and compact microscope system, multi-...

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Bibliographic Details
Published in:Lab on a chip 2017-01, Vol.17 (1), p.104-109
Main Authors: Meng, Xin, Huang, Huachuan, Yan, Keding, Tian, Xiaolin, Yu, Wei, Cui, Haoyang, Kong, Yan, Xue, Liang, Liu, Cheng, Wang, Shouyu
Format: Article
Language:English
Subjects:
Devices
Erythrocytes - cytology
Health care
Humans
Light
Mathematical analysis
Microscopes
Microscopy, Phase-Contrast - instrumentation
Microscopy, Phase-Contrast - methods
Poisson equation
Refractometry
Smartphone
Smartphones
Smear
Transport
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Summary:In order to realize high contrast imaging with portable devices for potential mobile healthcare, we demonstrate a hand-held smartphone based quantitative phase microscope using the transport of intensity equation method. With a cost-effective illumination source and compact microscope system, multi-focal images of samples can be captured by the smartphone's camera via manual focusing. Phase retrieval is performed using a self-developed Android application, which calculates sample phases from multi-plane intensities via solving the Poisson equation. We test the portable microscope using a random phase plate with known phases, and to further demonstrate its performance, a red blood cell smear, a Pap smear and monocot root and broad bean epidermis sections are also successfully imaged. Considering its advantages as an accurate, high-contrast, cost-effective and field-portable device, the smartphone based hand-held quantitative phase microscope is a promising tool which can be adopted in the future in remote healthcare and medical diagnosis.
ISSN:1473-0197
1473-0189
DOI:10.1039/c6lc01321j