Differentiating Pseudomonas sp. strain ADP cells in suspensions and biofilms using Raman spectroscopy and scanning electron microscopy

High quality spectra of Pseudomonas sp. strain ADP in the planktonic and biofilm state were obtained using Raman microspectroscopy. These spectra enabled the identification of key differences between free and biofilm cells in the fingerprint region of Raman spectra in the nucleic acid, carbohydrate,...

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Bibliographic Details
Published in:Analytical and bioanalytical chemistry 2017-02, Vol.409 (5), p.1441-1449
Main Authors: Henry, Victoria A., Jessop, Julie L. P., Peeples, Tonya L.
Format: Article
Language:English
Subjects:
Analytical Chemistry
Biochemistry
Biofilms
Bioremediation
Carbohydrates
Cell differentiation
Characterization and Evaluation of Materials
Chemistry
Chemistry and Materials Science
Extracellular matrix
Extraction
Food Science
Laboratory Medicine
Mathematical analysis
Methods
Microscopy, Electron, Scanning - methods
Monitoring/Environmental Analysis
Nucleic acids
Observations
Physiological aspects
Physiology
Pseudomonas
Pseudomonas - classification
Pseudomonas - metabolism
Pseudomonas - ultrastructure
Raman spectroscopy
Research Paper
Scanning electron microscopy
Scanning microscopy
Spectra
Spectroscopy
Spectrum analysis
Spectrum Analysis, Raman - methods
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Summary:High quality spectra of Pseudomonas sp. strain ADP in the planktonic and biofilm state were obtained using Raman microspectroscopy. These spectra enabled the identification of key differences between free and biofilm cells in the fingerprint region of Raman spectra in the nucleic acid, carbohydrate, and protein regions. Scanning electron microscopy (SEM) enabled detailed visualization of ADP biofilm with confirmation of associated extracellular matrix structure. Following extraction and Raman analysis of extracellular polymeric substances, Raman spectral differences between free and biofilm cells were largely attributed to the contribution of extracellular matrix components produced in mature biofilms. Raman spectroscopy complemented with SEM proves to be useful in distinguishing physiological properties among cells of the same species. Graphical Abstract Raman spectroscopy complemented with SEM proves to be useful in distinguishing physiological properties among cells of the same species
ISSN:1618-2642
1618-2650
DOI:10.1007/s00216-016-0077-9