Irradiation of myoglobin by intense, ultrashort laser pulses

We probe the interaction of myoglobin with intense, femtosecond laser pulses. Significant spectral differences are found between native and the irradiated myoglobin. These arise from the disruption of the heme prosthetic group: geometrical restructuring results in alteration of the oxidation state o...

Full description

Saved in:
Bibliographic Details
Published in:Applied physics. B, Lasers and optics Lasers and optics, 2016-10, Vol.122 (10), p.1-7, Article 253
Main Authors: Chelliah, Juliah J., Kumar, S. V. K., Dharmadhikari, Aditya K., Dharmadhikari, Jayashree A., Mathur, Deepak
Format: Article
Language:English
Subjects:
Applied physics
Backbone
Biomolecules
Disruption
Electron states
Engineering
Femtosecond
Femtosecond pulses
Irradiation
Lasers
Myoglobin
Myoglobins
Optical Devices
Optics
Oxidation
Photonics
Physical Chemistry
Physics
Physics and Astronomy
Polypeptides
Prostheses
Quantum Optics
Valence
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c349t-ee360998f40d753a01a09f22b66ee4add8a846550ee45b5bebe4230b9619a36c3
cites cdi_FETCH-LOGICAL-c349t-ee360998f40d753a01a09f22b66ee4add8a846550ee45b5bebe4230b9619a36c3
container_end_page 7
container_issue 10
container_start_page 1
container_title Applied physics. B, Lasers and optics
container_volume 122
creator Chelliah, Juliah J.
Kumar, S. V. K.
Dharmadhikari, Aditya K.
Dharmadhikari, Jayashree A.
Mathur, Deepak
description We probe the interaction of myoglobin with intense, femtosecond laser pulses. Significant spectral differences are found between native and the irradiated myoglobin. These arise from the disruption of the heme prosthetic group: geometrical restructuring results in alteration of the oxidation state of Fe (from its initial +3 state) which is found to be reversible on timescales of ~4–6 h. Measurements taken upon addition of OH scavengers establish the key role played by these radicals in the overall dynamics. Myoglobin remains intact upon intense field irradiation, demonstrating the structural robustness of the polypeptide backbone. Experiments utilizing intense, ultrashort laser pulses are expected to open new horizons for following, with high sensitivity, changes in the oxidation state, chemical environment, and electronic state of biomolecules in the aqueous phase.
doi_str_mv 10.1007/s00340-016-6526-5
format article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_1880020851</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>1880851796</sourcerecordid><originalsourceid>FETCH-LOGICAL-c349t-ee360998f40d753a01a09f22b66ee4add8a846550ee45b5bebe4230b9619a36c3</originalsourceid><addsrcrecordid>eNp1kE1LxDAQhoMouK7-AG8FLx6MTj6bgBdZ_FhY8KLnkLTp2qXbrEl72H9v1noQwbnMDDzvMDwIXRK4JQDlXQJgHDAQiaWgEosjNCOcUQyS62M0A80lpqQkp-gspQ3kkkrN0P0yRlu3dmhDX4Sm2O7Duguu7Qu3L9p-8H3yN8XYDdGmjxCHorPJx2I3dsmnc3TS2Dxc_PQ5en96fFu84NXr83LxsMIV43rA3jMJWquGQ10KZoFY0A2lTkrvua1rZRWXQkDehBPOO88pA6cl0ZbJis3R9XR3F8Pn6NNgtm2qfNfZ3ocxGaIUAAUlSEav_qCbMMY-f_dNZaTUMlNkoqoYUoq-MbvYbm3cGwLm4NNMPk32aQ4-jcgZOmVSZvu1j78u_xv6AnMIdvQ</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>1880851796</pqid></control><display><type>article</type><title>Irradiation of myoglobin by intense, ultrashort laser pulses</title><source>Springer Nature</source><creator>Chelliah, Juliah J. ; Kumar, S. V. K. ; Dharmadhikari, Aditya K. ; Dharmadhikari, Jayashree A. ; Mathur, Deepak</creator><creatorcontrib>Chelliah, Juliah J. ; Kumar, S. V. K. ; Dharmadhikari, Aditya K. ; Dharmadhikari, Jayashree A. ; Mathur, Deepak</creatorcontrib><description>We probe the interaction of myoglobin with intense, femtosecond laser pulses. Significant spectral differences are found between native and the irradiated myoglobin. These arise from the disruption of the heme prosthetic group: geometrical restructuring results in alteration of the oxidation state of Fe (from its initial +3 state) which is found to be reversible on timescales of ~4–6 h. Measurements taken upon addition of OH scavengers establish the key role played by these radicals in the overall dynamics. Myoglobin remains intact upon intense field irradiation, demonstrating the structural robustness of the polypeptide backbone. Experiments utilizing intense, ultrashort laser pulses are expected to open new horizons for following, with high sensitivity, changes in the oxidation state, chemical environment, and electronic state of biomolecules in the aqueous phase.</description><identifier>ISSN: 0946-2171</identifier><identifier>EISSN: 1432-0649</identifier><identifier>DOI: 10.1007/s00340-016-6526-5</identifier><language>eng</language><publisher>Berlin/Heidelberg: Springer Berlin Heidelberg</publisher><subject>Applied physics ; Backbone ; Biomolecules ; Disruption ; Electron states ; Engineering ; Femtosecond ; Femtosecond pulses ; Irradiation ; Lasers ; Myoglobin ; Myoglobins ; Optical Devices ; Optics ; Oxidation ; Photonics ; Physical Chemistry ; Physics ; Physics and Astronomy ; Polypeptides ; Prostheses ; Quantum Optics ; Valence</subject><ispartof>Applied physics. B, Lasers and optics, 2016-10, Vol.122 (10), p.1-7, Article 253</ispartof><rights>Springer-Verlag Berlin Heidelberg 2016</rights><rights>Copyright Springer Science &amp; Business Media 2016</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c349t-ee360998f40d753a01a09f22b66ee4add8a846550ee45b5bebe4230b9619a36c3</citedby><cites>FETCH-LOGICAL-c349t-ee360998f40d753a01a09f22b66ee4add8a846550ee45b5bebe4230b9619a36c3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Chelliah, Juliah J.</creatorcontrib><creatorcontrib>Kumar, S. V. K.</creatorcontrib><creatorcontrib>Dharmadhikari, Aditya K.</creatorcontrib><creatorcontrib>Dharmadhikari, Jayashree A.</creatorcontrib><creatorcontrib>Mathur, Deepak</creatorcontrib><title>Irradiation of myoglobin by intense, ultrashort laser pulses</title><title>Applied physics. B, Lasers and optics</title><addtitle>Appl. Phys. B</addtitle><description>We probe the interaction of myoglobin with intense, femtosecond laser pulses. Significant spectral differences are found between native and the irradiated myoglobin. These arise from the disruption of the heme prosthetic group: geometrical restructuring results in alteration of the oxidation state of Fe (from its initial +3 state) which is found to be reversible on timescales of ~4–6 h. Measurements taken upon addition of OH scavengers establish the key role played by these radicals in the overall dynamics. Myoglobin remains intact upon intense field irradiation, demonstrating the structural robustness of the polypeptide backbone. Experiments utilizing intense, ultrashort laser pulses are expected to open new horizons for following, with high sensitivity, changes in the oxidation state, chemical environment, and electronic state of biomolecules in the aqueous phase.</description><subject>Applied physics</subject><subject>Backbone</subject><subject>Biomolecules</subject><subject>Disruption</subject><subject>Electron states</subject><subject>Engineering</subject><subject>Femtosecond</subject><subject>Femtosecond pulses</subject><subject>Irradiation</subject><subject>Lasers</subject><subject>Myoglobin</subject><subject>Myoglobins</subject><subject>Optical Devices</subject><subject>Optics</subject><subject>Oxidation</subject><subject>Photonics</subject><subject>Physical Chemistry</subject><subject>Physics</subject><subject>Physics and Astronomy</subject><subject>Polypeptides</subject><subject>Prostheses</subject><subject>Quantum Optics</subject><subject>Valence</subject><issn>0946-2171</issn><issn>1432-0649</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2016</creationdate><recordtype>article</recordtype><recordid>eNp1kE1LxDAQhoMouK7-AG8FLx6MTj6bgBdZ_FhY8KLnkLTp2qXbrEl72H9v1noQwbnMDDzvMDwIXRK4JQDlXQJgHDAQiaWgEosjNCOcUQyS62M0A80lpqQkp-gspQ3kkkrN0P0yRlu3dmhDX4Sm2O7Duguu7Qu3L9p-8H3yN8XYDdGmjxCHorPJx2I3dsmnc3TS2Dxc_PQ5en96fFu84NXr83LxsMIV43rA3jMJWquGQ10KZoFY0A2lTkrvua1rZRWXQkDehBPOO88pA6cl0ZbJis3R9XR3F8Pn6NNgtm2qfNfZ3ocxGaIUAAUlSEav_qCbMMY-f_dNZaTUMlNkoqoYUoq-MbvYbm3cGwLm4NNMPk32aQ4-jcgZOmVSZvu1j78u_xv6AnMIdvQ</recordid><startdate>20161001</startdate><enddate>20161001</enddate><creator>Chelliah, Juliah J.</creator><creator>Kumar, S. V. K.</creator><creator>Dharmadhikari, Aditya K.</creator><creator>Dharmadhikari, Jayashree A.</creator><creator>Mathur, Deepak</creator><general>Springer Berlin Heidelberg</general><general>Springer Nature B.V</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7SP</scope><scope>7U5</scope><scope>8FD</scope><scope>H8D</scope><scope>L7M</scope></search><sort><creationdate>20161001</creationdate><title>Irradiation of myoglobin by intense, ultrashort laser pulses</title><author>Chelliah, Juliah J. ; Kumar, S. V. K. ; Dharmadhikari, Aditya K. ; Dharmadhikari, Jayashree A. ; Mathur, Deepak</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c349t-ee360998f40d753a01a09f22b66ee4add8a846550ee45b5bebe4230b9619a36c3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2016</creationdate><topic>Applied physics</topic><topic>Backbone</topic><topic>Biomolecules</topic><topic>Disruption</topic><topic>Electron states</topic><topic>Engineering</topic><topic>Femtosecond</topic><topic>Femtosecond pulses</topic><topic>Irradiation</topic><topic>Lasers</topic><topic>Myoglobin</topic><topic>Myoglobins</topic><topic>Optical Devices</topic><topic>Optics</topic><topic>Oxidation</topic><topic>Photonics</topic><topic>Physical Chemistry</topic><topic>Physics</topic><topic>Physics and Astronomy</topic><topic>Polypeptides</topic><topic>Prostheses</topic><topic>Quantum Optics</topic><topic>Valence</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Chelliah, Juliah J.</creatorcontrib><creatorcontrib>Kumar, S. V. K.</creatorcontrib><creatorcontrib>Dharmadhikari, Aditya K.</creatorcontrib><creatorcontrib>Dharmadhikari, Jayashree A.</creatorcontrib><creatorcontrib>Mathur, Deepak</creatorcontrib><collection>CrossRef</collection><collection>Electronics &amp; Communications Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Technology Research Database</collection><collection>Aerospace Database</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Applied physics. B, Lasers and optics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Chelliah, Juliah J.</au><au>Kumar, S. V. K.</au><au>Dharmadhikari, Aditya K.</au><au>Dharmadhikari, Jayashree A.</au><au>Mathur, Deepak</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Irradiation of myoglobin by intense, ultrashort laser pulses</atitle><jtitle>Applied physics. B, Lasers and optics</jtitle><stitle>Appl. Phys. B</stitle><date>2016-10-01</date><risdate>2016</risdate><volume>122</volume><issue>10</issue><spage>1</spage><epage>7</epage><pages>1-7</pages><artnum>253</artnum><issn>0946-2171</issn><eissn>1432-0649</eissn><abstract>We probe the interaction of myoglobin with intense, femtosecond laser pulses. Significant spectral differences are found between native and the irradiated myoglobin. These arise from the disruption of the heme prosthetic group: geometrical restructuring results in alteration of the oxidation state of Fe (from its initial +3 state) which is found to be reversible on timescales of ~4–6 h. Measurements taken upon addition of OH scavengers establish the key role played by these radicals in the overall dynamics. Myoglobin remains intact upon intense field irradiation, demonstrating the structural robustness of the polypeptide backbone. Experiments utilizing intense, ultrashort laser pulses are expected to open new horizons for following, with high sensitivity, changes in the oxidation state, chemical environment, and electronic state of biomolecules in the aqueous phase.</abstract><cop>Berlin/Heidelberg</cop><pub>Springer Berlin Heidelberg</pub><doi>10.1007/s00340-016-6526-5</doi><tpages>7</tpages></addata></record>
fulltext fulltext
identifier ISSN: 0946-2171
ispartof Applied physics. B, Lasers and optics, 2016-10, Vol.122 (10), p.1-7, Article 253
issn 0946-2171
1432-0649
language eng
recordid cdi_proquest_miscellaneous_1880020851
source Springer Nature
subjects Applied physics
Backbone
Biomolecules
Disruption
Electron states
Engineering
Femtosecond
Femtosecond pulses
Irradiation
Lasers
Myoglobin
Myoglobins
Optical Devices
Optics
Oxidation
Photonics
Physical Chemistry
Physics
Physics and Astronomy
Polypeptides
Prostheses
Quantum Optics
Valence
title Irradiation of myoglobin by intense, ultrashort laser pulses
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-02T05%3A49%3A55IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Irradiation%20of%20myoglobin%20by%20intense,%20ultrashort%20laser%20pulses&rft.jtitle=Applied%20physics.%20B,%20Lasers%20and%20optics&rft.au=Chelliah,%20Juliah%20J.&rft.date=2016-10-01&rft.volume=122&rft.issue=10&rft.spage=1&rft.epage=7&rft.pages=1-7&rft.artnum=253&rft.issn=0946-2171&rft.eissn=1432-0649&rft_id=info:doi/10.1007/s00340-016-6526-5&rft_dat=%3Cproquest_cross%3E1880851796%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c349t-ee360998f40d753a01a09f22b66ee4add8a846550ee45b5bebe4230b9619a36c3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=1880851796&rft_id=info:pmid/&rfr_iscdi=true