Multitargeting Gene Delivery Systems for Enhancing the Transfection of Endothelial Cells

Gene therapy demonstrates promising prospects on cardiovascular diseases. However, nonviral gene delivery system has relatively low transfection efficiency, especially for endothelial cells (ECs). Herein, typical cell‐penetrating peptide (TAT), nuclear localization signals (NLSs), and REDV functiona...

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Bibliographic Details
Published in:Macromolecular rapid communications. 2016-12, Vol.37 (23), p.1926-1931
Main Authors: Yang, Jing, Li, Qian, Yang, Xiao, Feng, Yakai, Ren, Xiangkui, Shi, Changcan, Zhang, Wencheng
Format: Article
Language:English
Subjects:
Cell-Penetrating Peptides - metabolism
Deoxyribonucleic acid
Efficiency
Encapsulation
Endothelial cells
Endothelial Cells - cytology
Endothelial Cells - metabolism
gene delivery
Genes
Humans
nanoparticles
Nuclear Localization Signals - metabolism
Peptides
Platforms
Polymers - chemical synthesis
Polymers - chemistry
Polymers - metabolism
REDV
targeting
Transfection - methods
Uptakes
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Summary:Gene therapy demonstrates promising prospects on cardiovascular diseases. However, nonviral gene delivery system has relatively low transfection efficiency, especially for endothelial cells (ECs). Herein, typical cell‐penetrating peptide (TAT), nuclear localization signals (NLSs), and REDV functional peptide have been used to prepare multitargeting complexes. These complexes exhibit higher transfection efficiency owing to the targeting sequences of REDV and NLSs as well as the cell‐penetrating function of TAT. The multifunction of the complexes provides high cell uptake, endo/lysosomal escape, and nucleus accumulation of the encapsulated DNA. Thus these multitargeting complexes can provide a potential platform for gene delivery, especially for EC transfection. Multitargeting complexes have been prepared from endothelial cell (EC)‐specific REDV peptide, typical cell‐penetrating peptide, and nuclear localization signals in order to specifically enhance the transfection efficiency. Owing to sequentially targeting and cell‐penetrate functions, these complexes provide a potential platform for gene delivery, especially for EC transfection with high cell uptake, endo/lysosomal escape, and nucleus accumulation of the encapsulated DNA.
ISSN:1022-1336
1521-3927
DOI:10.1002/marc.201600345