Cloning and Characterization of the Deoxyribonuclease sd[alpha] Gene from Streptococcus pyogenes

The pathogenesis of Streptococcus pyogenes infection, especially toxic shock-like syndrome (TSLS), is still not fully understood; however, the exoproteins have been considered to play a role. We analyzed the culture supernatant proteins (exoproteins) from a TSLS-related isolate belonging to M3 serot...

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Bibliographic Details
Published in:Current microbiology 2002-07, Vol.45 (1), p.13-17
Main Authors: Hasegawa, Tadao, Torii, Keizo, Hashikawa, Shinnosuke, Iinuma, Yoshitsugu, Ohta, Michio
Format: Article
Language:English
Subjects:
Bacteriology
Cloning
Medical research
Proteins
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Summary:The pathogenesis of Streptococcus pyogenes infection, especially toxic shock-like syndrome (TSLS), is still not fully understood; however, the exoproteins have been considered to play a role. We analyzed the culture supernatant proteins (exoproteins) from a TSLS-related isolate belonging to M3 serotype S. pyogenes by two-dimensional gel electrophoresis and characterized a single protein spot by using BLAST database. We cloned the gene of this protein and named it sdα, which was similar to the deoxyribonuclease (DNase) sdc of S. equisimilis. We showed that the recombinant protein from the sdα gene had DNase activity. By polymerase chain reaction, we found that the sdα gene was present in most clinically isolated S. pyogenes including TSLS-related isolates. We thus conclude that Sdα is a new DNase of S. pyogenes. [PUBLICATION ABSTRACT]
ISSN:0343-8651
1432-0991
DOI:10.1007/s00284-001-0042-4