Improving protein delivery of fibroblast growth factor-2 from bacterial inclusion bodies used as cell culture substrates

Bacterial inclusion bodies (IBs) have recently been used to generate biocompatible cell culture interfaces, with diverse effects on cultured cells such as cell adhesion enhancement, stimulation of cell growth or induction of mesenchymal stem cell differentiation. Additionally, novel applications of...

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Bibliographic Details
Published in:Acta biomaterialia 2014-03, Vol.10 (3), p.1354-1359
Main Authors: Seras-Franzoso, Joaquin, Peebo, Karl, García-Fruitós, Elena, Vázquez, Esther, Rinas, Ursula, Villaverde, Antonio
Format: Article
Language:English
Subjects:
Animals
Bacteria
Biological activity
Biotechnology
Cell Culture Techniques
Delivery systems
Drug delivery
Drug Delivery Systems
Escherichia coli
Escherichia coli - metabolism
Fibroblast Growth Factor 2 - pharmacology
Fibroblasts
HeLa Cells
Hep G2 Cells
Humans
Inclusion bodies
Inclusion Bodies - chemistry
Inclusion Bodies - drug effects
Inclusions
Mice
Nanomedicine
NIH 3T3 Cells
PC12 Cells
Protein release
Proteins
Rats
Surface functionalization
Topography
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Summary:Bacterial inclusion bodies (IBs) have recently been used to generate biocompatible cell culture interfaces, with diverse effects on cultured cells such as cell adhesion enhancement, stimulation of cell growth or induction of mesenchymal stem cell differentiation. Additionally, novel applications of IBs as sustained protein delivery systems with potential applications in regenerative medicine have been successfully explored. In this scenario, with IBs gaining significance in the biomedical field, the fine tuning of this functional biomaterial is crucial. In this work, the effect of temperature on fibroblast growth factor-2 (FGF-2) IB production and performance has been evaluated. FGF-2 was overexpressed in Escherichia coli at 25 and 37°C, producing IBs with differences in size, particle structure and biological activity. Cell culture topographies made with FGF-2 IBs biofabricated at 25°C showed higher levels of biological activity as well as a looser supramolecular structure, enabling a higher protein release from the particles. In addition, the controlled use of FGF-2 protein particles enabled the generation of functional topographies with multiple biological activities being effective on diverse cell types.
ISSN:1742-7061
1878-7568
DOI:10.1016/j.actbio.2013.12.021