A nanoporous membrane based impedance sensing platform for DNA sensing with gold nanoparticle amplification

Nucleic acids based detection has become increasingly important for biomedical diagnostics, environmental monitoring and food screening. In this paper, a nanoporous alumina membrane based impedance sensing platform with gold nanoparticle tags amplification was developed for DNA sensing. Single stran...

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Bibliographic Details
Published in:Sensors and actuators. B, Chemical Chemical, 2014-03, Vol.193, p.877-882
Main Authors: Ye, Wei Wei, Shi, Jing Yu, Chan, Chun Yu, Zhang, Yu, Yang, Mo
Format: Article
Language:English
Subjects:
Amplification
Blockage
Deoxyribonucleic acid
Detection
DNA sensor
Electrochemical impedance spectroscopy
Gold
Gold nanoparticles
Nanoporous alumina membrane
Nanostructure
Platforms
Silver enhancement
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Summary:Nucleic acids based detection has become increasingly important for biomedical diagnostics, environmental monitoring and food screening. In this paper, a nanoporous alumina membrane based impedance sensing platform with gold nanoparticle tags amplification was developed for DNA sensing. Single stranded DNA (ssDNA) probes were first covalently immobilized in nanopore walls via chemical linkers. DNA hybridization in the nanopores induced blockage in the nanopores, which could be detected by electrochemical impedance spectroscopy (EIS). The blockage signal could be further amplified by gold nanoparticles (AuNPs) conjugation and silver catalytic deposition method to reach a limit of detection (LOD) of 50pM. This platform is promising for simple, rapid and sensitive DNA detection.
ISSN:0925-4005
1873-3077
DOI:10.1016/j.snb.2013.09.115