Hierarchically Aligning 10 Legume Genomes Establishes a Family-Level Genomics Platform

• Published in: Plant physiology (Bethesda) 2017-05, Vol.174 (1), p.284-300
• Main Authors: Wang, Jinpeng, Sun, Pengchuan, Li, Yuxian, Liu, Yinzhe, Yu, Jigao, Ma, Xuelian, Sun, Sangrong, Yang, Nanshan, Xia, Ruiyan, Lei, Tianyu, Liu, Xiaojian, Jiao, Beibei, Xing, Yue, Ge, Weina, Wang, Li, Wang, Zhenyi, Song, Xiaoming, Yuan, Min, Guo, Di, Zhang, Lan, Zhang, Jiaqi, Jin, Dianchuan, Chen, Wei, Pan, Yuxin, Liu, Tao, Jin, Ling, Sun, Jinshuai, Yu, Jiaxiang, Cheng, Rui, Duan, Xueqian, Shen, Shaoqi, Qin, Jun, Zhang, Meng-chen, Paterson, Andrew H., Wang, Xiyin
• Format: Article
• Language: English
• Subjects: Chromosome Mapping; Evolution, Molecular; Fabaceae - classification; Fabaceae - genetics; Gene Duplication; GENES, DEVELOPMENT, AND EVOLUTION; Genes, Plant - genetics; Genome, Plant - genetics; Genomics - methods; Models, Genetic; Phylogeny; Polyploidy; Species Specificity
• ISSN: 0032-0889; 1532-2548
• DOI: 10.1104/pp.16.01981

Mainly due to their economic importance, genomes of 10 legumes, including soybean (Glycine max), wild peanut (Arachis duranensis and Arachis ipaensis), and barrel medic (Medicago truncatula), have been sequenced. However, a family-level comparative genomics analysis has been unavailable. With grape (Vitis vinifera) and selected legume genomes as outgroups, we managed to perform a hierarchical and event-related alignment of these genomes and deconvoluted layers of homologous regions produced by ancestral polyploidizations or speciations. Consequently, we illustrated genomic fractionation characterized by widespread gene losses after the polyploidizations. Notably, high similarity in gene retention between recently duplicated chromosomes in soybean supported the likely autopolyploidy nature of its tetraploid ancestor. Moreover, although most gene losses were nearly random, largely but not fully described by geometric distribution, we showed that polyploidization contributed divergently to the copy number variation of important gene families. Besides, we showed significantly divergent evolutionary levels among legumes and, by performing synonymous nucleotide substitutions at synonymous sites correction, redated major evolutionary events during their expansion. This effort laid a solid foundation for further genomics exploration in the legume research community and beyond. We describe only a tiny fraction of legume comparative genomics analysis that we performed; more information was stored in the newly constructed Legume Comparative Genomics Research Platform (www.legumegrp.org).