Unnatural amino acid mutagenesis in mapping ion channel function

Unnatural amino acid mutagenesis makes possible the site-specific incorporation of synthetic amino acids, enabling detailed structure–function studies as well as the incorporation of biophysical probes. This method has been adapted for use with heterologous expression in Xenopus oocytes, allowing ex...

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Bibliographic Details
Published in:Current opinion in neurobiology 2003-06, Vol.13 (3), p.264-270
Main Authors: Beene, Darren L, Dougherty, Dennis A, Lester, Henry A
Format: Article
Language:English
Subjects:
Amino Acids - chemistry
Amino Acids - physiology
Animals
Chromosome Mapping - methods
Humans
Ion Channels - chemistry
Ion Channels - physiology
Mutagenesis, Site-Directed - physiology
Oocytes
Structure-Activity Relationship
Xenopus
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Summary:Unnatural amino acid mutagenesis makes possible the site-specific incorporation of synthetic amino acids, enabling detailed structure–function studies as well as the incorporation of biophysical probes. This method has been adapted for use with heterologous expression in Xenopus oocytes, allowing experiments on ion channels.
ISSN:0959-4388
1873-6882
DOI:10.1016/S0959-4388(03)00068-0