Microbial production of 2-deoxyribose 5-phosphate from acetaldehyde and triosephosphate for the synthesis of 2'-deoxyribonucleosides

2-Deoxyribose 5-phosphate was produced from acetaldehyde and dihydroxyacetone phosphate via D-glyceraldehyde 3-phosphate by Klebsiella pneumoniae B-4-4 through deoxyriboaldolase- and triosephosphate isomerase-catalyzing reactions. Under the optimum conditions, 98.7 mM 2-deoxyribose 5-phosphate was p...

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Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2003-04, Vol.67 (4), p.933-936
Main Authors: Ogawa, J. (Kyoto Univ. (Japan)), Saito, K, Sakai, T, Horinouchi, N, Kawano, T, Matsumoto, S, Sasaki, M, Mikami, Y, Shimizu, S
Format: Article
Language:English
Subjects:
2-deoxyriobse 5-phosphate
2′-deoxyribonucleoside
ACETALDEHYDE
Acetaldehyde - metabolism
Biological and medical sciences
BIOSYNTHESIS
deoxyriboaldolase
DEOXYRIBONUCLEOSIDES
Deoxyribonucleosides - biosynthesis
Fundamental and applied biological sciences. Psychology
KLEBSIELLA PNEUMONIAE
Klebsiella pneumoniae - metabolism
MICROBIAL PROPERTIES
Pentosyltransferases - metabolism
PHOSPHATES
Phosphotransferases - metabolism
Ribosemonophosphates - biosynthesis
Sugar Phosphates - metabolism
triosephosphate
Trioses - metabolism
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Summary:2-Deoxyribose 5-phosphate was produced from acetaldehyde and dihydroxyacetone phosphate via D-glyceraldehyde 3-phosphate by Klebsiella pneumoniae B-4-4 through deoxyriboaldolase- and triosephosphate isomerase-catalyzing reactions. Under the optimum conditions, 98.7 mM 2-deoxyribose 5-phosphate was produced from 200 mM acetaldehyde and 117 mM dihydroxyacetone phosphate in 2 h with a molar yield of 84%. The 2-deoxyriobse 5-phosphate produced was directly transformed to 2′-deoxyribonucleoside by phosphopentomutase- and nucleoside phosphorylase-catalyzing reactions.
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.67.933