Role and regulation of TRP channels in neutrophil granulocytes

Members of the transient receptor potential (TRP) family for which mRNA can be demonstrated in neutrophil granulocytes with RT-PCR include TRPC6 (as only “short” TRP), TRPM2, TRPV1, TRPV2, TRPV5 and TRPV6. When these are analyzed in heterologous overexpression experiments, TRPM2 is the only cation c...

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Bibliographic Details
Published in:Cell calcium (Edinburgh) 2003-05, Vol.33 (5), p.533-540
Main Authors: Heiner, Inka, Eisfeld, Jörg, Lückhoff, Andreas
Format: Article
Language:English
Subjects:
Adenosine Diphosphate Ribose - pharmacology
ADP-ribose
Animals
Calcium Channels - metabolism
Granulocytes - drug effects
Granulocytes - metabolism
Humans
Hydrogen Peroxide - pharmacology
NAD - pharmacology
Neutrophil granulocytes
Neutrophils - drug effects
Neutrophils - metabolism
Oxidants - pharmacology
Signal Transduction
TRP channel
TRPC Cation Channels
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Summary:Members of the transient receptor potential (TRP) family for which mRNA can be demonstrated in neutrophil granulocytes with RT-PCR include TRPC6 (as only “short” TRP), TRPM2, TRPV1, TRPV2, TRPV5 and TRPV6. When these are analyzed in heterologous overexpression experiments, TRPM2 is the only cation channel with characteristic properties that can be used as fingerprint to provide functional evidence for its expression in neutrophil granulocytes. As cells transfected with TRPM2, neutrophil granulocytes display non-selective cation currents and typical channel activity evoked by intracellular ADP-ribose and NAD. Thus, stimulation of TRPM2 is likely to occur after activation of CD38 (producing ADP-ribose) and during the oxidative burst (enhancing the NAD concentration). This novel mode of cation entry regulation may be of particular importance for the response of granulocytes to chemoattractants. TRPV6 is a likely but not exclusive candidate as subunit of the channels mediating store-operated Ca 2+ entry (SOCE). Evidence for SOCE in granulocytes has been presented with the fura-2 technique but not with electrophysiological methods although Ca 2+-selective store-operated currents can be demonstrated in HL-60 cells, a cell culture model of neutrophil granulocytes.
ISSN:0143-4160
1532-1991
DOI:10.1016/S0143-4160(03)00058-7