Regulatory role of CCL5 (rs2280789) and CXCL10 (rs56061981) gene polymorphisms on intracellular CCL5 and CXCL10 expression in pulmonary tuberculosis

Abstract Genetic variations in chemokine genes influence the chemoattractive properties of T cells which may be associated with outcome of infections. In present study, we have investigated the regulatory role played by In1.1T/C (rs2280789) polymorphism of CCL5 and -135G/A (rs56061981) polymorphism...

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Bibliographic Details
Published in:Human immunology 2017-05, Vol.78 (5-6), p.430-434
Main Authors: Singh, Brijendra, Anbalagan, Selvaraj, Selvaraj, Paramasivam
Format: Article
Language:English
Subjects:
Adult
Allergy and Immunology
Cell Movement - genetics
Chemokine CCL5 - genetics
Chemokine CXCL10 - genetics
Female
Gene Expression Regulation
Genetic Predisposition to Disease
Genotype
Granuloma - genetics
Humans
Lymphocyte Activation
Male
Middle Aged
Mycobacterium tuberculosis - immunology
Polymorphism, Single Nucleotide
Th1 Cells - immunology
Th1 Cells - microbiology
Tuberculosis, Pulmonary - genetics
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Summary:Abstract Genetic variations in chemokine genes influence the chemoattractive properties of T cells which may be associated with outcome of infections. In present study, we have investigated the regulatory role played by In1.1T/C (rs2280789) polymorphism of CCL5 and -135G/A (rs56061981) polymorphism of CXCL10 gene on intracellular CCL5 and CXCL10 expression in T cells. Whole blood cell cultures were stimulated with culture filtrate antigen (CFA) and infected with live M. tuberculosis were used for intracellular CCL5 and CXCL10 expression using flow cytometry. Genotyping was performed using polymerase chain reaction based restriction fragment length polymorphism (PCR-RFLP). Significantly higher expression of CCL5 expressing CD3+ and CD3+CD8+ T cells were observed in HCs with In1.1TT genotype compared to C allele carrier (TT+TC) under unstimulated and CFA induced cultures (p
ISSN:0198-8859
1879-1166
DOI:10.1016/j.humimm.2017.03.008