Enhanced tenogenic differentiation and tendon- like tissue formation by CHIP overexpression in tendon-derived stem cells

The carboxyt terminus of Hsc70-interacting protein （CHIP, also known as STUB1） plays critical roles in the proliferation and differentiation of many types of cells. The potential function of CHIP in tendon-derived stem cells （TDSCs） remains largely unknown at present. Here, we investigated the effec...

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Bibliographic Details
Published in:Acta biochimica et biophysica Sinica 2017-04, Vol.49 (4), p.311-317
Main Authors: Han, Weifeng, Chen, Lei, Liu, Junpeng, Guo, Ai
Format: Article
Language:English
Subjects:
Adipogenesis - genetics
Animals
Blotting, Western
Cell Differentiation - genetics
Cell Lineage - genetics
Cell Proliferation - genetics
Cell Survival - genetics
Cell Transplantation - methods
Cells, Cultured
Gene Expression
Male
Mice, Nude
Microscopy, Confocal
Osteogenesis - genetics
Rats, Sprague-Dawley
Reverse Transcriptase Polymerase Chain Reaction
Stem Cells - cytology
Stem Cells - metabolism
Tendons - cytology
Tendons - metabolism
Transplantation, Heterologous
Ubiquitin-Protein Ligases - genetics
Ubiquitin-Protein Ligases - metabolism
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Summary:The carboxyt terminus of Hsc70-interacting protein （CHIP, also known as STUB1） plays critical roles in the proliferation and differentiation of many types of cells. The potential function of CHIP in tendon-derived stem cells （TDSCs） remains largely unknown at present. Here, we investigated the effects of CHIP on tenogenic differentiation of TDSCs via lentivirus-mediated overexpression. Forced expression of CHIP induced morphological changes and significantly enhanced cell prolif- eration, as well as tendon differentiation in vitro. Upon stimulation with differentiation induction medium, CHIP-overexpressing TDSCs displayed significant inhibition of differentiation into osteo- genic and adipogenic lineages. Subsequent implantation of TDSCs overexpressing CHIP with collagen sponges into nude mice induced a marked increase in ectopic tendon formation in vivo, compared with the control group. Our findings collectively suggest that CHIP is an important contributory factor to tenogenic tissue formation.
ISSN:1672-9145
1745-7270
DOI:10.1093/abbs/gmx005