Enhancing heterologous protection in pigs vaccinated with chimeric porcine reproductive and respiratory syndrome virus containing the full-length sequences of shuffled structural genes of multiple heterologous strains

Highlights • A chimera with full-length sequences of shuffled structural genes was generated. • The chimera induced neutralizing antibodies in pigs against heterologous strains. • The chimera conferred enhanced heterologous protection in pigs. • The chimera VR2385-S3456 is a good PRRSV vaccine candi...

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Bibliographic Details
Published in:Vaccine 2017-04, Vol.35 (18), p.2427-2434
Main Authors: Tian, Debin, Cao, Dianjun, Lynn Heffron, C, Yugo, Danielle M, Rogers, Adam J, Overend, Christopher, Matzinger, Shannon R, Subramaniam, Sakthivel, Opriessnig, Tanja, LeRoith, Tanya, Meng, Xiang-Jin
Format: Article
Language:English
Subjects:
Allergy and Immunology
Animals
Antibodies
Backbone
Chimeras
Cloning
Construction
Cross Protection
Deoxyribonucleic acid
DNA
DNA Shuffling
Economic importance
Gene sequencing
Genes
Genes, Viral
Genetic diversity
Heterologous strains
Hogs
Immunity, Heterologous
Morphology
Neutralizing
Nucleotide sequence
Porcine Reproductive and Respiratory Syndrome - prevention & control
Porcine reproductive and respiratory syndrome virus (PRRSV)
Porcine respiratory and reproductive syndrome virus - genetics
Porcine respiratory and reproductive syndrome virus - immunology
RFLP 1-7-4
Strains (organisms)
Swine
Vaccine
Vaccines
Vaccines, Synthetic - administration & dosage
Vaccines, Synthetic - genetics
Vaccines, Synthetic - immunology
Vaccines, Synthetic - isolation & purification
Viral Vaccines - administration & dosage
Viral Vaccines - genetics
Viral Vaccines - immunology
Viral Vaccines - isolation & purification
Viruses
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Summary:Highlights • A chimera with full-length sequences of shuffled structural genes was generated. • The chimera induced neutralizing antibodies in pigs against heterologous strains. • The chimera conferred enhanced heterologous protection in pigs. • The chimera VR2385-S3456 is a good PRRSV vaccine candidate.
ISSN:0264-410X
1873-2518
DOI:10.1016/j.vaccine.2017.03.046