Localized surface plasmon resonance-mediated fluorescence signals in plasmonic nanoparticle-quantum dot hybrids for ultrasensitive Zika virus RNA detection via hairpin hybridization assays

• Published in: Biosensors & bioelectronics 2017-08, Vol.94, p.513-522
• Main Authors: Adegoke, Oluwasesan, Morita, Masahiro, Kato, Tatsuya, Ito, Masahito, Suzuki, Tetsuro, Park, Enoch Y.
• Format: Article
• Language: English
• Subjects: Biosensing Techniques - methods; Fluorescence; Gold - chemistry; Humans; Metal Nanoparticles - chemistry; Molecular beacon; Nanoparticles - chemistry; Plasmonic nanoparticle; Quantum dot; Quantum Dots; RNA, Viral - chemistry; RNA, Viral - isolation & purification; Surface Plasmon Resonance; Zika virus; Zika Virus - chemistry; Zika Virus - isolation & purification; Zika Virus Infection - diagnosis; Zika Virus Infection - virology
• ISSN: 0956-5663; 1873-4235
• DOI: 10.1016/j.bios.2017.03.046

The current epidemic caused by the Zika virus (ZIKV) and the devastating effects of this virus on fetal development, which result in an increased incidence of congenital microcephaly symptoms, have prompted the World Health Organization (WHO) to declare the ZIKV a public health issue of global concern. Efficient probes that offer high detection sensitivity and specificity are urgently required to aid in the point-of-care treatment of the virus. In this study, we show that localized surface plasmon resonance (LSPR) signals from plasmonic nanoparticles (NPs) can be used to mediate the fluorescence signal from semiconductor quantum dot (Qdot) nanocrystals in a molecular beacon (MB) biosensor probe for ZIKV RNA detection. Four different plasmonic NPs functionalized with 3-mercaptopropionic acid (MPA), namely MPA-AgNPs, MPA-AuNPs, core/shell (CS) Au/AgNPs, and alloyed AuAgNPs, were synthesized and conjugated to L-glutathione-capped CdSeS alloyed Qdots to form the respective LSPR-mediated fluorescence nanohybrid. The concept of the plasmonic NP-Qdot-MB biosensor involves using LSPR from the plasmonic NPs to mediate a fluorescence signal to the Qdots, triggered by the hybridization of the target ZIKV RNA with the DNA loop sequence of the MB. The extent of the fluorescence enhancement based on ZIKV RNA detection was proportional to the LSPR-mediated fluorescence signal. The limits of detection (LODs) of the nanohybrids were as follows: alloyed AuAgNP-Qdot646-MB (1.7 copies/mL)) > CS Au/AgNP-Qdot646-MB (LOD =2.4 copies/mL) > AuNP-Qdot646-MB (LOD =2.9 copies/mL) > AgNP-Qdot646-MB (LOD =7.6 copies/mL). The LSPR-mediated fluorescence signal was stronger for the bimetallic plasmonic NP-Qdots than the single metallic plasmonic NP-Qdots. The plasmonic NP-Qdot-MB biosensor probes exhibited excellent selectivity toward ZIKV RNA and could serve as potential diagnostic probes for the point-of care detection of the virus. •Novel plasmonic nanoparticle-quantum dot (Qdot)-molecular beacon (MB) biosensor probes were developed.•Fluorescence intensity signal of the Qdots was mediated by a LSPR signal.•The LSPR-mediated Qdot-MB biosensor detected low concentrations of Zika virus RNA.•The LSPR-mediated Qdot-MB biosensor was selective for Zika virus RNA.