Molecular detection and characterization of picobirnaviruses in piglets with diarrhea in Thailand

Picobirnavirus (PBV) is a small, bi-segmented, double-stranded RNA virus. Taxonomically, the genus Picobirnavirus belongs to the Picobirnaviridae family. PBV infects a wide range of hosts and causes opportunistic infections, but its role in diarrheal disease remains unclear. To determine the prevale...

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Bibliographic Details
Published in:Archives of virology 2017-04, Vol.162 (4), p.1061-1066
Main Authors: Wilburn, Lauren, Yodmeeklin, Arpaporn, Kochjan, Pakawat, Saikruang, Wilaiporn, Kumthip, Kattareeya, Khamrin, Pattara, Maneekarn, Niwat
Format: Article
Language:English
Subjects:
Animals
Biomedical and Life Sciences
Biomedicine
Brief Report
Diarrhea
Diarrhea - veterinary
Diarrhea - virology
Feces - virology
Genetic diversity
Genomes
Hogs
Infections
Infectious Diseases
Medical Microbiology
Phylogenetics
Phylogeny
Picobirnaviridae
Picobirnavirus - classification
Picobirnavirus - genetics
Picobirnavirus - isolation & purification
Picobirnavirus - physiology
Reoviridae
RNA polymerase
RNA Virus Infections - veterinary
RNA Virus Infections - virology
Rotavirus
Swine
Swine Diseases - virology
Thailand
Thermal cycling
Virology
Viruses
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Summary:Picobirnavirus (PBV) is a small, bi-segmented, double-stranded RNA virus. Taxonomically, the genus Picobirnavirus belongs to the Picobirnaviridae family. PBV infects a wide range of hosts and causes opportunistic infections, but its role in diarrheal disease remains unclear. To determine the prevalence and genetic diversity of porcine PBVs in Northern Thailand, 380 fecal samples collected from diarrheic and non-diarrheic piglets, raised in 22 pig farms, were tested for the presence of PBV. Reverse-transcription PCR (RT-PCR) was performed using primer sets specific to the RNA-dependent RNA polymerase (RdRp) gene. PBV was detected in 86 of 265 (32.5%) diarrheic piglets and in 26 of 115 (22.6%) non-diarrheic piglets. All the PBV strains detected in this study belonged to genogroup I and a high proportion of PBV-positive piglets were co-infected with group A rotavirus (RVA) and bocavirus (BoV). Phylogenetic analysis of representative genogroup I strains revealed remarkably high similarity between strains; these formed a monophyletic cluster with 97-100% sequence identity in the RdRp gene. The strains were also closely related to genogroup I PBV Chinese porcine strain. The findings indicate that PBV infection is common in piglets with and without diarrhea in Northern Thailand.
ISSN:0304-8608
1432-8798
DOI:10.1007/s00705-016-3190-3