Nano‐Bio Engineered Carbon Dot‐Peptide Functionalized Water Dispersible Hyperbranched Polyurethane for Bone Tissue Regeneration

• Published in: Macromolecular bioscience 2017-03, Vol.17 (3), p.np-n/a
• Main Authors: Gogoi, Satyabrat, Maji, Somnath, Mishra, Debasish, Devi, K. Sanjana P., Maiti, Tapas Kumar, Karak, Niranjan
• Format: Article
• Language: English
• Subjects: Biocompatible Materials - administration & dosage; Biocompatible Materials - chemistry; bio‐nanohybrid; Bone Regeneration - drug effects; bone tissue engineering; Bones; Carbon; Carbon - chemistry; carbon dot; Cell Adhesion - drug effects; Cell Differentiation - drug effects; Cell Proliferation - drug effects; Hep G2 Cells; Humans; Nanocomposites; Nanocomposites - chemistry; Osteoblasts - drug effects; peptide; Peptides - administration & dosage; Peptides - chemistry; polyurethane; Polyurethanes - administration & dosage; Polyurethanes - chemistry; Tannins - chemistry; Tissue Engineering; Tissue Scaffolds - chemistry
• ISSN: 1616-5187; 1616-5195
• DOI: 10.1002/mabi.201600271

The present study delves into a combined bio‐nano‐macromolecular approach for bone tissue engineering. This approach relies on the properties of an ideal scaffold material imbued with all the chemical premises required for fostering cellular growth and differentiation. A tannic acid based water dispersible hyperbranched polyurethane is fabricated with bio‐nanohybrids of carbon dot and four different peptides (viz. SVVYGLR, PRGDSGYRGDS, IPP, and CGGKVGKACCVPTKLSPISVLYK) to impart target specific in vivo bone healing ability. This polymeric bio‐nanocomposite is blended with 10 wt% of gelatin and examined as a non‐invasive delivery vehicle. In vitro assessment of the developed polymeric system reveals good osteoblast adhesion, proliferation, and differentiation. Aided by this panel of peptides, the polymeric bio‐nanocomposite exhibits in vivo ectopic bone formation ability. The study on in vivo mineralization and vascularization reveals the occurrence of calcification and blood vessel formation. Thus, the study demonstrates carbon dot/peptide functionalized hyperbranched polyurethane gel for bone tissue engineering application. Water dispersible hyperbranched polyurethane is conjugated with bio‐nano‐hybrid of carbon dot and RGD sequenced peptides to obtain a target specific multifunctional system with excellent in vitro and in vivo osteogenic activity.