Hymenobacter knuensis sp. nov., Isolated From River Water

• Published in: Current microbiology 2017-04, Vol.74 (4), p.515-521
• Main Authors: Ten, Leonid N., Lee, Jae-Jin, Lee, Yeon-Hee, Park, Su-Jin, Lee, Seung-Yeol, Park, Sangkyu, Lee, Dae Sung, Kang, In-Kyu, Kim, Myung Kyum, Jung, Hee-Young
• Format: Article
• Language: English
• Subjects: Bacteroidetes - classification; Bacteroidetes - genetics; Bacteroidetes - physiology; Base Composition - genetics; Biomedical and Life Sciences; Biotechnology; Cytophagaceae; Deoxyribonucleic acid; DNA; DNA, Bacterial - genetics; Genotype; Gram-negative bacteria; Life Sciences; Microbiology; Phosphatidylethanolamines - metabolism; Phylogeny; Rivers; Rivers - microbiology; RNA, Ribosomal, 16S - genetics; Taxonomy; Water; Water Microbiology
• ISSN: 0343-8651; 1432-0991
• DOI: 10.1007/s00284-017-1216-z

A Gram-stain-negative, non-motile, non-spore-forming, rod-shaped, aerobic bacterial strain, designated 16F7C-2 T , was isolated from the Han River, South Korea, and was characterized taxonomically using a polyphasic approach. Comparative 16S rRNA gene sequence analysis showed that strain 16F7C-2 T belonged to the family Cytophagaceae in the phylum Bacteroidetes and was most closely related to Hymenobacter algoricola VUG-A23a T (98.3%) and Hymenobacter fastidiosus VUG-A124 T (97.7%). The G + C content of the genomic DNA of strain 16F7C-2 T was 63.4 mol%. The detection of menaquinone MK-7 as the predominant respiratory quinone; a fatty acid profile with summed feature 3 (C 16:1 ω 7 c /C 16:1 ω 6 c ; 19.5%), summed feature 4 (C 17:1 iso I/C 17:1 anteiso B; 15.9%), C 15:0 iso (12.6%), C 16:1 ω 5 c (10.5%), and C 15:0 anteiso (10.4%) as the major components; and a polar lipid profile with phosphatidylethanolamine as the major component also supported the affiliation of strain 16F7C-2 T to the genus Hymenobacter . The DNA–DNA relatedness between strain 16F7C-2 T and H. algoricola JCM 17214 T and H. fastidiosus JCM17224 T were 45.2 ± 5.8 and 40.3 ± 2.9%, respectively, clearly showing that the isolate constitutes a new genospecies. Strain 16F7C-2 T could be clearly differentiated from its closest neighbors on the basis of its phenotypic, genotypic, and chemotaxonomic features. Therefore, strain 16F7C-2 T represents a novel species of the genus Hymenobacter , for which the name Hymenobacter knuensis sp. nov. is proposed. The type strain is 16F7C-2 T (=KCTC 52538 T  = JCM 31814 T ).